Knowledge Resource Center for Ecological Environment in Arid Area
DOI | 10.1007/s11033-011-1017-6 |
Molecular cloning of a stress-responsive aldehyde dehydrogenase gene ScALDH21 from the desiccation-tolerant moss Syntrichia caninervis and its responses to different stresses | |
Yang, Honglan1; Zhang, Daoyuan1; Wang, Jiancheng1; Wood, Andrew J.2; Zhang, Yuanming1![]() | |
通讯作者 | Zhang, Daoyuan |
来源期刊 | MOLECULAR BIOLOGY REPORTS
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ISSN | 0301-4851 |
EISSN | 1573-4978 |
出版年 | 2012 |
卷号 | 39期号:3页码:2645-2652 |
英文摘要 | Aldehyde dehydrogenases (ALDHs) are key enzymes of abiotic stress-tolerance in a variety of organisms. The ALDH gene superfamily in eukaryotes has identified 22 protein families based upon sequence identity. ALDH21 is unique to mosses and represented by a single transcript gene in the desiccation-tolerant moss Tortula ruralis. We describe the cloning and characterization of an ALDH21 homologue from Syntrichia caninervis (ScALDH21), an extremely desiccation-tolerant moss found in deserts of Central Asia. The ScALDH21 cDNA is 1,452 bp and encodes a deduced polypeptide of 483 amino acids (53 kDa), approximately 97% identical to T. ruralis ALDH21 (TrALDH21A). The ScALDH21 gene was sub-cloned into pET26b(+) and expressed in Escherichia coli (Rosetta) to determine the peptides function in response to desiccation and salinity. Quantitative RT-PCR was used to analyze steady-state mRNA amounts in response to Abscisic acid (ABA) and desiccation. ScALDH21 transcript levels increased significantly in response to both desiccation and ABA. In the transgenic E. coli, ScALDH21 protein could be induced under the salinity and desiccation stress and was more abundant within salt-treated gametophores relative to control tissue. The data suggest that ScALDH21 participates in the stress-resistant pathways and plays an important role in response to desiccation and salinity stresses. |
英文关键词 | ScALDH21 Cloning Syntrichia caninervis Expression Desiccation Abiotic stress |
类型 | Article |
语种 | 英语 |
国家 | Peoples R China ; USA |
收录类别 | SCI-E |
WOS记录号 | WOS:000301107800065 |
WOS关键词 | PHYSCOMITRELLA-PATENS ; ARABIDOPSIS-THALIANA ; TOBACCO PLANTS ; SUPERFAMILY ; EXPRESSION ; DROUGHT ; SPECIFICITY |
WOS类目 | Biochemistry & Molecular Biology |
WOS研究方向 | Biochemistry & Molecular Biology |
来源机构 | 中国科学院新疆生态与地理研究所 |
资源类型 | 期刊论文 |
条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/174074 |
作者单位 | 1.Chinese Acad Sci, Xinjiang Inst Ecol & Geog, Key Lab Biogeog & Bioresource Arid Land, Urumqi 830011, Xinjiang, Peoples R China; 2.So Illinois Univ, Dept Plant Biol, Carbondale, IL 62901 USA |
推荐引用方式 GB/T 7714 | Yang, Honglan,Zhang, Daoyuan,Wang, Jiancheng,et al. Molecular cloning of a stress-responsive aldehyde dehydrogenase gene ScALDH21 from the desiccation-tolerant moss Syntrichia caninervis and its responses to different stresses[J]. 中国科学院新疆生态与地理研究所,2012,39(3):2645-2652. |
APA | Yang, Honglan,Zhang, Daoyuan,Wang, Jiancheng,Wood, Andrew J.,&Zhang, Yuanming.(2012).Molecular cloning of a stress-responsive aldehyde dehydrogenase gene ScALDH21 from the desiccation-tolerant moss Syntrichia caninervis and its responses to different stresses.MOLECULAR BIOLOGY REPORTS,39(3),2645-2652. |
MLA | Yang, Honglan,et al."Molecular cloning of a stress-responsive aldehyde dehydrogenase gene ScALDH21 from the desiccation-tolerant moss Syntrichia caninervis and its responses to different stresses".MOLECULAR BIOLOGY REPORTS 39.3(2012):2645-2652. |
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