Arid
DOI10.1007/s11033-011-1017-6
Molecular cloning of a stress-responsive aldehyde dehydrogenase gene ScALDH21 from the desiccation-tolerant moss Syntrichia caninervis and its responses to different stresses
Yang, Honglan1; Zhang, Daoyuan1; Wang, Jiancheng1; Wood, Andrew J.2; Zhang, Yuanming1
通讯作者Zhang, Daoyuan
来源期刊MOLECULAR BIOLOGY REPORTS
ISSN0301-4851
EISSN1573-4978
出版年2012
卷号39期号:3页码:2645-2652
英文摘要

Aldehyde dehydrogenases (ALDHs) are key enzymes of abiotic stress-tolerance in a variety of organisms. The ALDH gene superfamily in eukaryotes has identified 22 protein families based upon sequence identity. ALDH21 is unique to mosses and represented by a single transcript gene in the desiccation-tolerant moss Tortula ruralis. We describe the cloning and characterization of an ALDH21 homologue from Syntrichia caninervis (ScALDH21), an extremely desiccation-tolerant moss found in deserts of Central Asia. The ScALDH21 cDNA is 1,452 bp and encodes a deduced polypeptide of 483 amino acids (53 kDa), approximately 97% identical to T. ruralis ALDH21 (TrALDH21A). The ScALDH21 gene was sub-cloned into pET26b(+) and expressed in Escherichia coli (Rosetta) to determine the peptides function in response to desiccation and salinity. Quantitative RT-PCR was used to analyze steady-state mRNA amounts in response to Abscisic acid (ABA) and desiccation. ScALDH21 transcript levels increased significantly in response to both desiccation and ABA. In the transgenic E. coli, ScALDH21 protein could be induced under the salinity and desiccation stress and was more abundant within salt-treated gametophores relative to control tissue. The data suggest that ScALDH21 participates in the stress-resistant pathways and plays an important role in response to desiccation and salinity stresses.


英文关键词ScALDH21 Cloning Syntrichia caninervis Expression Desiccation Abiotic stress
类型Article
语种英语
国家Peoples R China ; USA
收录类别SCI-E
WOS记录号WOS:000301107800065
WOS关键词PHYSCOMITRELLA-PATENS ; ARABIDOPSIS-THALIANA ; TOBACCO PLANTS ; SUPERFAMILY ; EXPRESSION ; DROUGHT ; SPECIFICITY
WOS类目Biochemistry & Molecular Biology
WOS研究方向Biochemistry & Molecular Biology
来源机构中国科学院新疆生态与地理研究所
资源类型期刊论文
条目标识符http://119.78.100.177/qdio/handle/2XILL650/174074
作者单位1.Chinese Acad Sci, Xinjiang Inst Ecol & Geog, Key Lab Biogeog & Bioresource Arid Land, Urumqi 830011, Xinjiang, Peoples R China;
2.So Illinois Univ, Dept Plant Biol, Carbondale, IL 62901 USA
推荐引用方式
GB/T 7714
Yang, Honglan,Zhang, Daoyuan,Wang, Jiancheng,et al. Molecular cloning of a stress-responsive aldehyde dehydrogenase gene ScALDH21 from the desiccation-tolerant moss Syntrichia caninervis and its responses to different stresses[J]. 中国科学院新疆生态与地理研究所,2012,39(3):2645-2652.
APA Yang, Honglan,Zhang, Daoyuan,Wang, Jiancheng,Wood, Andrew J.,&Zhang, Yuanming.(2012).Molecular cloning of a stress-responsive aldehyde dehydrogenase gene ScALDH21 from the desiccation-tolerant moss Syntrichia caninervis and its responses to different stresses.MOLECULAR BIOLOGY REPORTS,39(3),2645-2652.
MLA Yang, Honglan,et al."Molecular cloning of a stress-responsive aldehyde dehydrogenase gene ScALDH21 from the desiccation-tolerant moss Syntrichia caninervis and its responses to different stresses".MOLECULAR BIOLOGY REPORTS 39.3(2012):2645-2652.
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