Arid
DOI10.1016/j.jplph.2012.04.007
Expression of AtSAP5 in cotton up-regulates putative stress-responsive genes and improves the tolerance to rapidly developing water deficit and moderate heat stress
Hozain, Moh’d1; Abdelmageed, Haggag3; Lee, Joohyun3; Kang, Miyoung3; Fokar, Mohamed2; Allen, Randy D.3; Holaday, A. Scott1
通讯作者Holaday, A. Scott
来源期刊JOURNAL OF PLANT PHYSIOLOGY
ISSN0176-1617
出版年2012
卷号169期号:13页码:1261-1270
英文摘要

The regulation of gene expression is a key factor in plant acclimation to stress, and it is thought that manipulation of the expression of critical stress-responsive genes should ultimately provide increased protection against abiotic stress. The aim of this study was to test the hypothesis that the ectopic expression of the AtSAP5 (AT3G12630) gene in transgenic cotton (Gossypium hirsutum, cv. Coker 312) will improve tolerance to drought and heat stress by up-regulating the expression of endogenous stress-responsive genes. The SAP5 gene is a member of the stress-associated family of genes that encode proteins containing A20/AN1 zinc finger domains. Under non-stressful conditions, cotton plants that expressed the AtSAP5 gene showed elevated expression of at least four genes normally induced during water deficit or heat stress. The rate of net CO2 assimilation A for three of four transgenic lines tested was less sensitive to rapidly developing water deficit over 4 d than untransformed wild-type plants, but the recovery of A following drought was not significantly affected. The enhanced protection of photosynthesis during drought was determined to be primarily at the biochemical level, since the extent of stomatal closure was not significantly different for all genotypes. Expression of AtSAP5 resulted in the complete protection of photosystem (PS)11 complexes from photodamage at mid-day after 4 d of drought, whereas wild-type plants experienced a 20% decline in active photosystem II (PSII) complexes. In addition, enhanced protection of seedling growth and leaf viability was associated with the expression of AtSAP5. Since A for the transgenic plants was significantly more heat tolerant than A for wild-type plants, we conclude that ectopic expression of SAP genes is a potentially viable approach to improving carbon gain and productivity for cotton grown in semi-arid regions with severe drought and heat stress. (c) 2012 Elsevier GmbH. All rights reserved.


英文关键词Drought stress Gossypium Heat stress Photosynthesis Stress-responsive genes
类型Article
语种英语
国家USA
收录类别SCI-E
WOS记录号WOS:000309246700005
WOS关键词GOSSYPIUM-HIRSUTUM L. ; HIGH LIGHT-INTENSITY ; GENOME-WIDE ANALYSIS ; FIELD-GROWN COTTON ; RUBISCO ACTIVASE ; HIGH-TEMPERATURE ; C-3 PLANTS ; TRANSGENIC TOBACCO ; ANTIOXIDANT ENZYME ; CONFERS TOLERANCE
WOS类目Plant Sciences
WOS研究方向Plant Sciences
资源类型期刊论文
条目标识符http://119.78.100.177/qdio/handle/2XILL650/173811
作者单位1.Texas Tech Univ, Dept Biol Sci, Lubbock, TX 79409 USA;
2.Texas Tech Univ, Ctr Biotechnol & Genom, Lubbock, TX 79409 USA;
3.Oklahoma State Univ, Inst Agr Biosci, Ardmore, OK 73401 USA
推荐引用方式
GB/T 7714
Hozain, Moh’d,Abdelmageed, Haggag,Lee, Joohyun,et al. Expression of AtSAP5 in cotton up-regulates putative stress-responsive genes and improves the tolerance to rapidly developing water deficit and moderate heat stress[J],2012,169(13):1261-1270.
APA Hozain, Moh’d.,Abdelmageed, Haggag.,Lee, Joohyun.,Kang, Miyoung.,Fokar, Mohamed.,...&Holaday, A. Scott.(2012).Expression of AtSAP5 in cotton up-regulates putative stress-responsive genes and improves the tolerance to rapidly developing water deficit and moderate heat stress.JOURNAL OF PLANT PHYSIOLOGY,169(13),1261-1270.
MLA Hozain, Moh’d,et al."Expression of AtSAP5 in cotton up-regulates putative stress-responsive genes and improves the tolerance to rapidly developing water deficit and moderate heat stress".JOURNAL OF PLANT PHYSIOLOGY 169.13(2012):1261-1270.
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