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DOI | 10.1016/j.chroma.2012.06.095 |
Development of a sensitive and robust liquid chromatography coupled with tandem mass spectrometry and a pressurized liquid extraction for the determination of aflatoxins and ochratoxin A in animal derived foods | |
Chen, Dongmei; Cao, Xiaoqin; Tao, Yanfei; Wu, Qinghua; Pan, Yuanhu; Huang, Lingli; Wang, Xu; Wang, Yulian; Peng, Dapeng; Liu, Zhenli; Yuan, Zonghui1 | |
通讯作者 | Yuan, Zonghui |
来源期刊 | JOURNAL OF CHROMATOGRAPHY A
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ISSN | 0021-9673 |
出版年 | 2012 |
卷号 | 1253页码:110-119 |
英文摘要 | A liquid chromatography-tandem mass spectrometry (LC-MS/MS) with a pressurized liquid extraction (PLE) was developed for the simultaneous determination of aflatoxins B-1, B-2, G(1), G(2), M-1 and M-2, and ochratoxin A in the muscle, liver, kidney and fat of swine, bovine and sheep, muscle and liver of chicken, muscle and skin of fish, as well as in hen eggs and dairy milk. Samples were extracted with PLE and cleaned-up with solid phase extraction (SPE) on HLB cartridges. The optimum extraction conditions were obtained as a 11 ml ASE cell, acetonitrile/hexane as the extraction solvent, 1500 psi, 100 degrees C, a 5 min static time and a 60% flush volume. A cheaper and widely used SPE column (Oasis HLB) was applied during clean up. The detection and quantification of the 7 mycotoxins were performed by a reversed-phase liquid chromatography coupled with electrospray ionization triple quadrupole mass spectrometry (LC/ESI-MS/MS). The limits of detection defined as CC alpha varied from 0.07 mu g/kg to 0.59 mu g/kg. The recoveries of spiked samples from 0.25 mu g/kg to 1 mu g/kg ranged from 68.3% to 105.7% with the relative standard deviations of less than 17.6%. Performances of the whole analytical procedure met the criteria established by the European Commission for mass spectrometric detection. (C) 2012 Elsevier B.V. All rights reserved. |
英文关键词 | Aflatoxins Ochratoxin A Pressurized liquid extraction LC-MS/MS Animal derived foods |
类型 | Article |
语种 | 英语 |
国家 | Peoples R China |
收录类别 | SCI-E |
WOS记录号 | WOS:000307793700013 |
WOS关键词 | ACCELERATED SOLVENT-EXTRACTION ; FLUORESCENCE DETECTION ; POSTCOLUMN DERIVATIZATION ; CONFIRMATORY METHOD ; LC-MS/MS ; MILK ; MYCOTOXINS ; RESIDUES ; FUSARIUM ; CEREALS |
WOS类目 | Biochemical Research Methods ; Chemistry, Analytical |
WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
资源类型 | 期刊论文 |
条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/173395 |
作者单位 | 1.Huazhong Agr Univ, MOA Key Lab Detect Vet Drug Residues, Wuhan 430070, Hubei, Peoples R China; 2.Huazhong Agr Univ, Natl Reference Lab Vet Drug Residues HZAU, Wuhan 430070, Hubei, Peoples R China |
推荐引用方式 GB/T 7714 | Chen, Dongmei,Cao, Xiaoqin,Tao, Yanfei,et al. Development of a sensitive and robust liquid chromatography coupled with tandem mass spectrometry and a pressurized liquid extraction for the determination of aflatoxins and ochratoxin A in animal derived foods[J],2012,1253:110-119. |
APA | Chen, Dongmei.,Cao, Xiaoqin.,Tao, Yanfei.,Wu, Qinghua.,Pan, Yuanhu.,...&Yuan, Zonghui.(2012).Development of a sensitive and robust liquid chromatography coupled with tandem mass spectrometry and a pressurized liquid extraction for the determination of aflatoxins and ochratoxin A in animal derived foods.JOURNAL OF CHROMATOGRAPHY A,1253,110-119. |
MLA | Chen, Dongmei,et al."Development of a sensitive and robust liquid chromatography coupled with tandem mass spectrometry and a pressurized liquid extraction for the determination of aflatoxins and ochratoxin A in animal derived foods".JOURNAL OF CHROMATOGRAPHY A 1253(2012):110-119. |
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