Arid
DOI10.1016/j.chroma.2012.06.095
Development of a sensitive and robust liquid chromatography coupled with tandem mass spectrometry and a pressurized liquid extraction for the determination of aflatoxins and ochratoxin A in animal derived foods
Chen, Dongmei; Cao, Xiaoqin; Tao, Yanfei; Wu, Qinghua; Pan, Yuanhu; Huang, Lingli; Wang, Xu; Wang, Yulian; Peng, Dapeng; Liu, Zhenli; Yuan, Zonghui1
通讯作者Yuan, Zonghui
来源期刊JOURNAL OF CHROMATOGRAPHY A
ISSN0021-9673
出版年2012
卷号1253页码:110-119
英文摘要

A liquid chromatography-tandem mass spectrometry (LC-MS/MS) with a pressurized liquid extraction (PLE) was developed for the simultaneous determination of aflatoxins B-1, B-2, G(1), G(2), M-1 and M-2, and ochratoxin A in the muscle, liver, kidney and fat of swine, bovine and sheep, muscle and liver of chicken, muscle and skin of fish, as well as in hen eggs and dairy milk. Samples were extracted with PLE and cleaned-up with solid phase extraction (SPE) on HLB cartridges. The optimum extraction conditions were obtained as a 11 ml ASE cell, acetonitrile/hexane as the extraction solvent, 1500 psi, 100 degrees C, a 5 min static time and a 60% flush volume. A cheaper and widely used SPE column (Oasis HLB) was applied during clean up. The detection and quantification of the 7 mycotoxins were performed by a reversed-phase liquid chromatography coupled with electrospray ionization triple quadrupole mass spectrometry (LC/ESI-MS/MS). The limits of detection defined as CC alpha varied from 0.07 mu g/kg to 0.59 mu g/kg. The recoveries of spiked samples from 0.25 mu g/kg to 1 mu g/kg ranged from 68.3% to 105.7% with the relative standard deviations of less than 17.6%. Performances of the whole analytical procedure met the criteria established by the European Commission for mass spectrometric detection. (C) 2012 Elsevier B.V. All rights reserved.


英文关键词Aflatoxins Ochratoxin A Pressurized liquid extraction LC-MS/MS Animal derived foods
类型Article
语种英语
国家Peoples R China
收录类别SCI-E
WOS记录号WOS:000307793700013
WOS关键词ACCELERATED SOLVENT-EXTRACTION ; FLUORESCENCE DETECTION ; POSTCOLUMN DERIVATIZATION ; CONFIRMATORY METHOD ; LC-MS/MS ; MILK ; MYCOTOXINS ; RESIDUES ; FUSARIUM ; CEREALS
WOS类目Biochemical Research Methods ; Chemistry, Analytical
WOS研究方向Biochemistry & Molecular Biology ; Chemistry
资源类型期刊论文
条目标识符http://119.78.100.177/qdio/handle/2XILL650/173395
作者单位1.Huazhong Agr Univ, MOA Key Lab Detect Vet Drug Residues, Wuhan 430070, Hubei, Peoples R China;
2.Huazhong Agr Univ, Natl Reference Lab Vet Drug Residues HZAU, Wuhan 430070, Hubei, Peoples R China
推荐引用方式
GB/T 7714
Chen, Dongmei,Cao, Xiaoqin,Tao, Yanfei,et al. Development of a sensitive and robust liquid chromatography coupled with tandem mass spectrometry and a pressurized liquid extraction for the determination of aflatoxins and ochratoxin A in animal derived foods[J],2012,1253:110-119.
APA Chen, Dongmei.,Cao, Xiaoqin.,Tao, Yanfei.,Wu, Qinghua.,Pan, Yuanhu.,...&Yuan, Zonghui.(2012).Development of a sensitive and robust liquid chromatography coupled with tandem mass spectrometry and a pressurized liquid extraction for the determination of aflatoxins and ochratoxin A in animal derived foods.JOURNAL OF CHROMATOGRAPHY A,1253,110-119.
MLA Chen, Dongmei,et al."Development of a sensitive and robust liquid chromatography coupled with tandem mass spectrometry and a pressurized liquid extraction for the determination of aflatoxins and ochratoxin A in animal derived foods".JOURNAL OF CHROMATOGRAPHY A 1253(2012):110-119.
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