Knowledge Resource Center for Ecological Environment in Arid Area
DOI | 10.3390/ijms13066944 |
Reference Gene Selection in the Desert Plant Eremosparton songoricum | |
Li, Xiao-Shuang1,2; Yang, Hong-Lan1; Zhang, Dao-Yuan1; Zhang, Yuan-Ming1; Wood, Andrew J.3 | |
通讯作者 | Zhang, Dao-Yuan |
来源期刊 | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES |
ISSN | 1661-6596 |
EISSN | 1422-0067 |
出版年 | 2012 |
卷号 | 13期号:6页码:6944-6963 |
英文摘要 | Eremosparton songoricum (Litv.) Vass. (E. songoricum) is a rare and extremely drought-tolerant desert plant that holds promise as a model organism for the identification of genes associated with water deficit stress. Here, we cloned and evaluated the expression of eight candidate reference genes using quantitative real-time reverse transcriptase polymerase chain reactions. The expression of these candidate reference genes was analyzed in a diverse set of 20 samples including various E. songoricum plant tissues exposed to multiple environmental stresses. GeNorm analysis indicated that expression stability varied between the reference genes in the different experimental conditions, but the two most stable reference genes were sufficient for normalization in most conditions. EsEF and Esa-TUB were sufficient for various stress conditions, EsEF and EsACT were suitable for samples of differing germination stages, and EsGAPDHand EsUBQ were most stable across multiple adult tissue samples. The Es18S gene was unsuitable as a reference gene in our analysis. In addition, the expression level of the drought-stress related transcription factor EsDREB2 verified the utility of E. songoricum reference genes and indicated that no single gene was adequate for normalization on its own. This is the first systematic report on the selection of reference genes in E. songoricum, and these data will facilitate future work on gene expression in this species. |
英文关键词 | Eremosparton songoricum quantitative real-time PCR reference genes geNorm validation |
类型 | Article |
语种 | 英语 |
国家 | Peoples R China ; USA |
收录类别 | SCI-E |
WOS记录号 | WOS:000306188700026 |
WOS关键词 | TIME RT-PCR ; POLYMERASE-CHAIN-REACTION ; BINDING TRANSCRIPTION FACTOR ; INTERNAL REFERENCE GENES ; FUNCTIONAL-CHARACTERIZATION ; SALT TOLERANCE ; EXPRESSION ; NORMALIZATION ; IDENTIFICATION ; VALIDATION |
WOS类目 | Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary |
WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
来源机构 | 中国科学院新疆生态与地理研究所 |
资源类型 | 期刊论文 |
条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/172966 |
作者单位 | 1.Chinese Acad Sci, Xinjiang Inst Ecol & Geog, Key Lab Biogeog & Bioresource Arid Land, Urumqi 830011, Peoples R China; 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China; 3.So Illinois Univ, Dept Plant Biol, Carbondale, IL 62901 USA |
推荐引用方式 GB/T 7714 | Li, Xiao-Shuang,Yang, Hong-Lan,Zhang, Dao-Yuan,et al. Reference Gene Selection in the Desert Plant Eremosparton songoricum[J]. 中国科学院新疆生态与地理研究所,2012,13(6):6944-6963. |
APA | Li, Xiao-Shuang,Yang, Hong-Lan,Zhang, Dao-Yuan,Zhang, Yuan-Ming,&Wood, Andrew J..(2012).Reference Gene Selection in the Desert Plant Eremosparton songoricum.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,13(6),6944-6963. |
MLA | Li, Xiao-Shuang,et al."Reference Gene Selection in the Desert Plant Eremosparton songoricum".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 13.6(2012):6944-6963. |
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