Arid
DOI10.1002/gcc.20952
Mapping of homozygous deletions in verified esophageal adenocarcinoma cell lines and xenografts
Boonstra, Jurjen J.1,2; van Marion, Ronald1; Douben, Hannie J. C. W.3; Lanchbury, Jerry S.4; Timms, Kirsten M.4; Abkevich, Victor4; Tilanus, Hugo W.2; de Klein, Annelies3; Dinjens, Winand N. M.1
通讯作者Boonstra, Jurjen J.
来源期刊GENES CHROMOSOMES & CANCER
ISSN1045-2257
EISSN1098-2264
出版年2012
卷号51期号:3页码:272-282
英文摘要

Human esophageal adenocarcinoma (EAC) cell lines and xenografts are powerful tools in the search for genetic alterations because these models are composed of pure human cancer cell populations without admixture of normal human cells. In particular detection of homozygous deletions (HDs) is easier using these pure populations of cancer cells. Identification of HDs could potentially lead to the subsequent identification of new tumor suppressor genes (TSGs) involved in esophageal adenocarcinogenesis. Genome wide single nucleotide polymorphism (SNP) arrays were used to identify HDs in 10 verified EAC cell lines and nine EAC xenografts. In total, 61 HDs (range 16 per sample) were detected and confirmed by polymerase chain reaction. Besides HDs observed in common fragile genomic regions (n = 26), and gene deserts (n = 8), 27 HDs were located in gene-containing regions. HDs were noted for known TSGs, including CDKN2A, SMAD4 and CDH3/CDH1. Twenty-two new chromosomal regions were detected harboring potentially new TSGs involved in EAC carcinogenesis. Two of these regions of homozygous loss, encompassing the ITGAV and RUNX1 gene, were detected in multiple samples indicating a potential role in the carcinogenesis of EAC. To exclude culturing artifacts, these last two deletions were confirmed by fluorescent in situ hybridization in the primary tumors of which the involved cell lines and xenografts were derived. In summary, in this report we describe the identification of HDs in a series of verified EAC cell lines and xenografts. The deletions documented here are a step forward identifying the key genes involved in EAC development. (c) 2011 Wiley Periodicals, Inc.


类型Article
语种英语
国家Netherlands ; USA
收录类别SCI-E
WOS记录号WOS:000298880000008
WOS关键词NUCLEOTIDE POLYMORPHISM ARRAYS ; BARRETTS-ESOPHAGUS ; E-CADHERIN ; PROSTATE-CANCER ; SUPPRESSOR GENE ; GASTRIC-CANCER ; IN-VIVO ; GENOME ; PROGRESSION ; MUTATIONS
WOS类目Oncology ; Genetics & Heredity
WOS研究方向Oncology ; Genetics & Heredity
资源类型期刊论文
条目标识符http://119.78.100.177/qdio/handle/2XILL650/172464
作者单位1.Univ Med Ctr Rotterdam, Erasmus MC, Josephine Nefkens Inst, Dept Pathol, NL-3000 CA Rotterdam, Netherlands;
2.Univ Med Ctr Rotterdam, Erasmus MC, Dept Surg, NL-3000 CA Rotterdam, Netherlands;
3.Univ Med Ctr Rotterdam, Erasmus MC, Dept Clin Genet, NL-3000 CA Rotterdam, Netherlands;
4.Myriad Genet Inc, Salt Lake City, UT USA
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Boonstra, Jurjen J.,van Marion, Ronald,Douben, Hannie J. C. W.,et al. Mapping of homozygous deletions in verified esophageal adenocarcinoma cell lines and xenografts[J],2012,51(3):272-282.
APA Boonstra, Jurjen J..,van Marion, Ronald.,Douben, Hannie J. C. W..,Lanchbury, Jerry S..,Timms, Kirsten M..,...&Dinjens, Winand N. M..(2012).Mapping of homozygous deletions in verified esophageal adenocarcinoma cell lines and xenografts.GENES CHROMOSOMES & CANCER,51(3),272-282.
MLA Boonstra, Jurjen J.,et al."Mapping of homozygous deletions in verified esophageal adenocarcinoma cell lines and xenografts".GENES CHROMOSOMES & CANCER 51.3(2012):272-282.
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