干旱区生态环境知识资源中心

A method combining solid-phase extraction and high-performance liquid chromatography (SPE-HPLC) has been developed for analysis of ten rare ginsenosides including 20(R)-Rh-1, 20(S)-Rh-1, 20(R)-Rg(3), 20(S)-Rg(3), Rg(5), Rk(1), Rg(6), F-4, Rk(3), and Rh-4 in three kinds of injection (Shenfu, Shenmai, and Shengmai). Waters Oasis HLB SPE columns were used to clean and enrich the sample. An Eclipse XDB-C-18 column was used to separate the analytes, with water and acetonitrile as mobile phase components under gradient elution conditions at 30 A degrees C. There were good linear correlations between the signals measured and the concentrations of the ten analytes (r (2) a parts per thousand yen 0.9996). Intraday and interday precision were both better than 2.19% and average recovery ranged from 95.25 to 108.83%. The proposed method was successfully applied to determination of the ten analytes in different batches of the injections and the results indicated the method is simple, rapid, and accurate. The proposed method could be used for quality control during manufacture of the injections.