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DOI | 10.1016/j.apsb.2012.07.003 |
Simultaneous quantitation of lamivudine, zidovudine and nevirapine in human plasma by liquid chromatography-tandem mass spectrometry and application to a pharmacokinetic study | |
Matta, Murali Krishna1; Pilli, Nageswara Rao1; Inamadugu, Jaswanth Kumar2; Burugula, Laxminarayana1; Rao, Seshagiri J. V. L. N.3 | |
通讯作者 | Rao, Seshagiri J. V. L. N. |
来源期刊 | ACTA PHARMACEUTICA SINICA B
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ISSN | 2211-3835 |
EISSN | 2211-3843 |
出版年 | 2012 |
卷号 | 2期号:5页码:472-480 |
英文摘要 | A rapid and sensitive LC MS/MS method for the simultaneous quantitation of lamivudine, zidovudine and nevirapine in human plasma using abacavir as internal standard has been developed and validated. The analytes and IS were extracted from plasma by solid phase extraction using Oasis HLB cartridges and separated on a Hypurity Advance C18 column using a mixture of acetonitrile:0.10/. formic acid (76:24, v/v) at a flow rate of 0.8 mL/min. Detection involved an API4000 LC MS/MS with electrospray ionization in the positive ion mode and multiplereaction monitoring for analysis. The method was validated according to FDA guidelines and shown to provide intra- and interday precision and accuracy within acceptable limits in a run time of only 3.5 min. The method was successfully applied to a pharmacokinetic study involving a single oral administration of a combination tablet to human male volunteers. (C) 2012 Institute of Materia Medica, Chinese Academy of Medical Sciences and Chinese Pharmaceutical Association. Production and hosting by Elsevier B.V. All rights reserved. |
英文关键词 | Lamivudine Zidovudine Nevirapine Solid phase extraction LC MS/MS Pharmacokinetics |
类型 | Article |
语种 | 英语 |
国家 | India |
收录类别 | SCI-E |
WOS记录号 | WOS:000413439600006 |
WOS类目 | Pharmacology & Pharmacy |
WOS研究方向 | Pharmacology & Pharmacy |
资源类型 | 期刊论文 |
条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/170939 |
作者单位 | 1.Jawaharlal Nehru Technol Univ, Coll Pharmaceut Sci, Hyderabad 500085, Andhra Pradesh, India; 2.Sri Venkateswara Univ, Dept Chem, Analyt & Environm Chem Div, Tirupati 517502, Andhra Pradesh, India; 3.Andhra Univ, Coll Pharmaceut Sci, Visakhapatnam 530003, Andhra Pradesh, India |
推荐引用方式 GB/T 7714 | Matta, Murali Krishna,Pilli, Nageswara Rao,Inamadugu, Jaswanth Kumar,et al. Simultaneous quantitation of lamivudine, zidovudine and nevirapine in human plasma by liquid chromatography-tandem mass spectrometry and application to a pharmacokinetic study[J],2012,2(5):472-480. |
APA | Matta, Murali Krishna,Pilli, Nageswara Rao,Inamadugu, Jaswanth Kumar,Burugula, Laxminarayana,&Rao, Seshagiri J. V. L. N..(2012).Simultaneous quantitation of lamivudine, zidovudine and nevirapine in human plasma by liquid chromatography-tandem mass spectrometry and application to a pharmacokinetic study.ACTA PHARMACEUTICA SINICA B,2(5),472-480. |
MLA | Matta, Murali Krishna,et al."Simultaneous quantitation of lamivudine, zidovudine and nevirapine in human plasma by liquid chromatography-tandem mass spectrometry and application to a pharmacokinetic study".ACTA PHARMACEUTICA SINICA B 2.5(2012):472-480. |
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