Knowledge Resource Center for Ecological Environment in Arid Area
DOI | 10.1590/S0100-67622011000400013 |
ESTIMATION OF OUT-CROSSING IN Spondias tuberosa Arruda (ANACARDIACEAE) POPULATION BY USING AFLP MARKER | |
Fernandes Santos, Carlos Antonio; de Oliveira, Viseldo Ribeiro; Rodrigues, Marciene Amorim; Coelho Ribeiro, Hugo Leonardo; Drumond, Marcos Antonio | |
通讯作者 | Fernandes Santos, Carlos Antonio |
来源期刊 | REVISTA ARVORE
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ISSN | 0100-6762 |
出版年 | 2011 |
卷号 | 35期号:3页码:691-697 |
英文摘要 | Umbu tree is one of the most important species in the Brazilian semi-arid region due to its ability to produce fruit in water stress environments. The objective of this work was to estimate outcrossing rates in the S. tuberosa ((t) over cap), by considering the observed and expected frequency of heterozygous in the mother and descent population (M1), respectively, and the estimate of multilocus (M2), to direct programs of conservation and genetic improvement of the species. Samples of DNA extracted from a maternal population with 96 plants established in 1991, at Petrolina, PE, as well as the DNA from one descent from each maternal plant were analyzed for the 16 polymorphic AFLP bands obtained from AAA_CTG and AAA_CTC primer combinations. In M1, it was considered the frequency of heterozygote estimated by the square root of 1 minus the AFLP fragment absence frequency, for the maternal population (observed frequency) and also for the descent population (expected frequency), whereas in the M2, the estimate was obtained by the multilocus estimate ((t) over capm). The (t) over cap estimates of M1 ranged from 1.74 to 0.50, with mean value of 1.063. Because values of (t) over cap above 1.0 are biologically inadequate, it was demonstrated that M1 was not appropriate to estimate (t) over cap. Frequencies of pollen and ovule for 15 AFLP loci were the same, suggesting good adjustment to the mixed mating model in M2. The (t) over cap rate obtained by M2 was 0.719, which was close to previous estimates with isoenzymes obtained in other umbu tree populations. Results point that umbu tree is predominantly an outcrossing species and there is the need for broad samples in order to preserve the genetic variability of this species. |
英文关键词 | Heterozygous MLTR and Outcrossing rate |
类型 | Article |
语种 | 葡萄牙语 |
国家 | Brazil |
收录类别 | SCI-E |
WOS记录号 | WOS:000300568400013 |
WOS关键词 | BREEDING POPULATION ; SEMIARID REGION |
WOS类目 | Forestry |
WOS研究方向 | Forestry |
资源类型 | 期刊论文 |
条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/170360 |
作者单位 | EMBRAPA, Empresa Brasileira Pesquisa Agr, Planaltina, DF, Brazil |
推荐引用方式 GB/T 7714 | Fernandes Santos, Carlos Antonio,de Oliveira, Viseldo Ribeiro,Rodrigues, Marciene Amorim,et al. ESTIMATION OF OUT-CROSSING IN Spondias tuberosa Arruda (ANACARDIACEAE) POPULATION BY USING AFLP MARKER[J],2011,35(3):691-697. |
APA | Fernandes Santos, Carlos Antonio,de Oliveira, Viseldo Ribeiro,Rodrigues, Marciene Amorim,Coelho Ribeiro, Hugo Leonardo,&Drumond, Marcos Antonio.(2011).ESTIMATION OF OUT-CROSSING IN Spondias tuberosa Arruda (ANACARDIACEAE) POPULATION BY USING AFLP MARKER.REVISTA ARVORE,35(3),691-697. |
MLA | Fernandes Santos, Carlos Antonio,et al."ESTIMATION OF OUT-CROSSING IN Spondias tuberosa Arruda (ANACARDIACEAE) POPULATION BY USING AFLP MARKER".REVISTA ARVORE 35.3(2011):691-697. |
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