Arid
DOI10.1590/S0100-67622011000400013
ESTIMATION OF OUT-CROSSING IN Spondias tuberosa Arruda (ANACARDIACEAE) POPULATION BY USING AFLP MARKER
Fernandes Santos, Carlos Antonio; de Oliveira, Viseldo Ribeiro; Rodrigues, Marciene Amorim; Coelho Ribeiro, Hugo Leonardo; Drumond, Marcos Antonio
通讯作者Fernandes Santos, Carlos Antonio
来源期刊REVISTA ARVORE
ISSN0100-6762
出版年2011
卷号35期号:3页码:691-697
英文摘要

Umbu tree is one of the most important species in the Brazilian semi-arid region due to its ability to produce fruit in water stress environments. The objective of this work was to estimate outcrossing rates in the S. tuberosa ((t) over cap), by considering the observed and expected frequency of heterozygous in the mother and descent population (M1), respectively, and the estimate of multilocus (M2), to direct programs of conservation and genetic improvement of the species. Samples of DNA extracted from a maternal population with 96 plants established in 1991, at Petrolina, PE, as well as the DNA from one descent from each maternal plant were analyzed for the 16 polymorphic AFLP bands obtained from AAA_CTG and AAA_CTC primer combinations. In M1, it was considered the frequency of heterozygote estimated by the square root of 1 minus the AFLP fragment absence frequency, for the maternal population (observed frequency) and also for the descent population (expected frequency), whereas in the M2, the estimate was obtained by the multilocus estimate ((t) over capm). The (t) over cap estimates of M1 ranged from 1.74 to 0.50, with mean value of 1.063. Because values of (t) over cap above 1.0 are biologically inadequate, it was demonstrated that M1 was not appropriate to estimate (t) over cap. Frequencies of pollen and ovule for 15 AFLP loci were the same, suggesting good adjustment to the mixed mating model in M2. The (t) over cap rate obtained by M2 was 0.719, which was close to previous estimates with isoenzymes obtained in other umbu tree populations. Results point that umbu tree is predominantly an outcrossing species and there is the need for broad samples in order to preserve the genetic variability of this species.


英文关键词Heterozygous MLTR and Outcrossing rate
类型Article
语种葡萄牙语
国家Brazil
收录类别SCI-E
WOS记录号WOS:000300568400013
WOS关键词BREEDING POPULATION ; SEMIARID REGION
WOS类目Forestry
WOS研究方向Forestry
资源类型期刊论文
条目标识符http://119.78.100.177/qdio/handle/2XILL650/170360
作者单位EMBRAPA, Empresa Brasileira Pesquisa Agr, Planaltina, DF, Brazil
推荐引用方式
GB/T 7714
Fernandes Santos, Carlos Antonio,de Oliveira, Viseldo Ribeiro,Rodrigues, Marciene Amorim,et al. ESTIMATION OF OUT-CROSSING IN Spondias tuberosa Arruda (ANACARDIACEAE) POPULATION BY USING AFLP MARKER[J],2011,35(3):691-697.
APA Fernandes Santos, Carlos Antonio,de Oliveira, Viseldo Ribeiro,Rodrigues, Marciene Amorim,Coelho Ribeiro, Hugo Leonardo,&Drumond, Marcos Antonio.(2011).ESTIMATION OF OUT-CROSSING IN Spondias tuberosa Arruda (ANACARDIACEAE) POPULATION BY USING AFLP MARKER.REVISTA ARVORE,35(3),691-697.
MLA Fernandes Santos, Carlos Antonio,et al."ESTIMATION OF OUT-CROSSING IN Spondias tuberosa Arruda (ANACARDIACEAE) POPULATION BY USING AFLP MARKER".REVISTA ARVORE 35.3(2011):691-697.
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