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In order to evaluate seed yield and oil percentage of winter canola cultivars in semi-arid region of Iran, an experiment was conducted at Research Farm of Isfahan Agriculture Research Station, Mahyar in 2010. A split plot arrangement based on randomized complete block design with three replications was used. The main plots included four irrigation levels (Complete irrigation, disruption of irrigation from stem elongation stage, disruption of irrigation from flowering stage and disruption of irrigation from pod formation stage), and sub-plots were six canola cultivars, namely, OKAPI, MODENA, LICORD, ZARFAM, SLM046 and OPERA). Irrigation had significant influence on number of days from plantation until stem elongation, and complete flowering, duration of flowering and physiological maturation. Plant height, the number of secondary branches, 1000-seed weight, seed yield and oil percentage were significantly influenced by disruption of irrigation. Cultivar also had significant effect on all experimental characteristics, expect of the number of pods per plant. Irrigation and cultivar interaction also had significant influence on the number of secondary branches, 1000-seed weight and oil percentage. In all experimental characteristics, by increasing drought stress, the significant decrease was shown. Therefore, complete irrigation is needed at all growth stages to maintain and enhance crop yield. Irrigation is critical during flowering phase in order to improve seed yield and oil percentage. The highest seed yield and oil percentage were obtained in Modena.