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The volatile oils obtained from Tanacetum nubigenum Wallich ex DC. (Asteraceae), an alpine cold desert plant collected from four different habitats of Garhwal and Kumaon Himalaya of Uttarakhand (India) were subjected to GC-MS analysis in order to determine the variation of concentration in their constituents. Plants collected from Gothing showed selin-11-en-4-alpha-ol (10.3%) as a major constituent. Methyl acetopyronone (9.5%), Borneol (19.8%), p-menthene-1-ol (11.7%), 1,8-cineole (10.9%) and cis-piperitol (10.9%) were obtained in a high concentration in the plants collected from Burphu. Similarly, bornyl acetate (38.1%) and borneol (19.5%) were the major constituents in Milam Glacier sample, whereas in the sample collected from Badrinath, 1,8-cineole (50.5%) was the most abundant component followed by beta-eudesmol (11.2%). The volatile oils of all the samples constituted lesser amount of monoterpene (7.5%-14.3%) and sesquiterpene hydrocarbons (0.3%-10.2%) whereas oxygenated monoterpenes contained larger amount (48.4-83.8%). In all the populations variations in their constituents were observed, although the species was same.