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A micropropagation method for Jaal (Salvadora persica)-a tree of arid horticulture and forestry has been developed. Nodal segments of fresh shoot sprouts originated from axillary buds obtained from a plant around 35-40 years old lopped plant were used as explants for establishment of in vitro cultures. Surface-sterilized explants produced optimum number of shoots through activation of axillary buds on Murashige and Skoog’s (MS) medium containing 8.88 mu M BA (6-benzyladenine) + additives (25 mgl(-1) each of adenine sulphate, arginine, citric acid, 50 mgl(-1) ascorbic acid). The shoot multiplication was influenced by the successive transfer of the mother explants for 4-5 passages. The maximum number (23.1 +/- A 0.73 shoots per explant) of shoots were regenerated on MS supplemented with 1.11 mu M BA + 1.16 mu M Kn (Kinetin) + 0.54 mu M NAA (alpha-naphthalene acetic acid). About 90% shoots pulse-treated with a combination of 2460.27 mu M Indole-3-butyric acid (IBA) + 494.56 mu M NOA (2-naphthoxy acetic acid) were rooted ex vitro on soilrite within 15-18 days. Over 80% cloned plantlets were hardened successfully in a green house and transferred to polybag/pots.