Knowledge Resource Center for Ecological Environment in Arid Area
DOI | 10.1007/s12033-010-9357-3 |
Isolation of High-Quality RNA from Reaumuria soongorica, a Desert Plant Rich in Secondary Metabolites | |
Wang, Xiaohua1; Xiao, Honglang1; Chen, Guoxiong2; Zhao, Xin2; Huang, Chenghong2; Chen, Cuiyun2; Wang, Fang1 | |
通讯作者 | Xiao, Honglang |
来源期刊 | MOLECULAR BIOTECHNOLOGY |
ISSN | 1073-6085 |
出版年 | 2011 |
卷号 | 48期号:2页码:165-172 |
英文摘要 | RNA isolation is a prerequisite for the study of the molecular mechanisms of stress tolerance in the desert plant Reaumuria soongorica, an extreme xeric semi-shrub. However, R. soongorica that contains high levels of secondary metabolites that co-precipitate with RNA, making RNA isolation difficult. Here the authors propose a new protocol suitable for isolating high-quality RNA from the leaves of R. soongorica. Based on a CTAB method described by Liu et al., the protocol has been improved as follows: the samples were ground with PVPP to effectively inhibit the oxidation of phenolics, contaminating DNA was removed with DNase I, and NaAc was used along with ethanol for precipitation to enhance the RNA yield and shorten the precipitation time. Gel electrophoresis and spectrophotometric analysis indicated that this isolation method provides RNA with no DNA contamination. Moreover, the yield (183.79 +/- A 40.36 mu g/g) and quality were superior to those using the method of Liu et al., which yields RNA with significant DNA contamination at 126.30 +/- A 29.43 mu g/g. Gene amplification showed that the RNA obtained using this protocol is suitable for use in downstream molecular procedures. This method was found to work equally well for isolating RNA from other desert plants. Thus, it is likely to be widely applicable. |
英文关键词 | CTAB Desert plants RNA isolation Reaumuria soongorica Secondary metabolites |
类型 | Article |
语种 | 英语 |
国家 | Peoples R China |
收录类别 | SCI-E |
WOS记录号 | WOS:000289800000008 |
WOS关键词 | POLYSACCHARIDE-RICH ; INEXPENSIVE METHOD ; EFFICIENT METHOD ; NUCLEIC-ACIDS ; TISSUES ; EXTRACTION ; POLYPHENOLS ; PROTOCOL ; SEEDS ; DNA |
WOS类目 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
WOS研究方向 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
来源机构 | 中国科学院西北生态环境资源研究院 |
资源类型 | 期刊论文 |
条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/169694 |
作者单位 | 1.Chinese Acad Sci, Key Lab Ecohydrol & Inland River Basin, Cold & Arid Reg Environm & Engn Res Inst, Lanzhou 730000, Peoples R China; 2.Chinese Acad Sci, Lab Plant Ecophysiol & Biotechnol, Cold & Arid Reg Environm & Engn Res Inst, Lanzhou 730000, Peoples R China |
推荐引用方式 GB/T 7714 | Wang, Xiaohua,Xiao, Honglang,Chen, Guoxiong,et al. Isolation of High-Quality RNA from Reaumuria soongorica, a Desert Plant Rich in Secondary Metabolites[J]. 中国科学院西北生态环境资源研究院,2011,48(2):165-172. |
APA | Wang, Xiaohua.,Xiao, Honglang.,Chen, Guoxiong.,Zhao, Xin.,Huang, Chenghong.,...&Wang, Fang.(2011).Isolation of High-Quality RNA from Reaumuria soongorica, a Desert Plant Rich in Secondary Metabolites.MOLECULAR BIOTECHNOLOGY,48(2),165-172. |
MLA | Wang, Xiaohua,et al."Isolation of High-Quality RNA from Reaumuria soongorica, a Desert Plant Rich in Secondary Metabolites".MOLECULAR BIOTECHNOLOGY 48.2(2011):165-172. |
条目包含的文件 | 条目无相关文件。 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。