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A non-motile, rod-shaped, red-pigmented bacterium, designated strain BZ33r(T), was isolated from soil of an industrial site. Cells stained Gram-negative and were aerobic and psychrophilic, showing good growth at 1-20 degrees C. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain BZ33r(T) was related to members of the genus Hymenobacter and had highest sequence similarity with Hymenobacter aerophilus DSM 13606(T) (97.5%). The predominant cellular fatty acids were anteiso-C(15:0) (20.3%), summed feature 3 (C(16:1)omega 7c and/or iso-C(15:0) 2-OH; 20.2%), iso-C(15:0) (20.0%), summed feature 4 (iso-C(17:1) I and/or anteiso-C(17:1) B; 8.2%) and C(16:1)omega 5c (7.9%). Strain BZ33r(T) had MK-7 as the major menaquinone. The polyamine pattern contained sym-homospermidine as the major compound with moderate amounts of spermidine. Phosphatidylethanolamine, three unknown aminophospholipids, two unknown aminolipids, two unknown glycolipids and five unknown polar lipids were present in the polar lipid profile. The G+C content of the DNA was 61.6 mol%. On the basis of phenotypic characteristics, phylogenetic analysis and DNA-DNA relatedness data, strain BZ33r(T) is considered to represent a novel species of the genus Hymenobacter, for which the name Hymenobacter psychrophilus sp. nov. is proposed. The type strain is BZ33r(T) (=DSM 22290(T) =CGMCC 1.8975(T) =LMG 25548(T)).