Knowledge Resource Center for Ecological Environment in Arid Area
CRYOPROTECTIVE EFFECT OF AN INSECT ANTIFREEZE PROTEIN MPAFP 698 AND ITS MUTANTS FROM THE DESERT BEETLE MICRODERA PUNCTIPENNIS | |
Jiang, Min; Ma, Ji; Qiu, Liming | |
通讯作者 | Ma, Ji |
来源期刊 | CRYOLETTERS |
ISSN | 0143-2044 |
EISSN | 1742-0644 |
出版年 | 2011 |
卷号 | 32期号:5页码:436-446 |
英文摘要 | An insect antifreeze protein, MpAFP698, from the desert beetle Microdera punctipennis, shares 77% similarity with TmTHP (YL-3) from Tenebrio molitor. The predicted tertiary structure for MpAFP698 was modeled as a regular beta-helix with TCT motifs arrayed to form the putative ice-binding surface. This model was validated via site-directed mutagenesis. The results suggest that the desert beetle in central Asia has evolved antifreeze proteins similar to those from North America continent. In an additional study, the recombinant MpAFP698 and its mutants were expressed in Escherichia coli strain BL21 (DE3). The thermal hysteresis activity of MpAFP698 was 1.73 degrees C at 1.0 mg/ml. MpAFP698 was also tested by in vitro antifreeze activity assay to evaluate its cryoprotective effect at -20 degrees C. MpAFP698 displays high cryoprotective effect on bacteria cells at freezing temperatures. |
英文关键词 | antifreeze protein Microdera punctipennis site-directed mutagenesis thermal hysteresis |
类型 | Article |
语种 | 英语 |
国家 | Peoples R China |
收录类别 | SCI-E |
WOS记录号 | WOS:000296681200008 |
WOS关键词 | ICE-BINDING FACE ; COLD TOLERANCE ; GLYCOPROTEINS ; ARTHROPODS ; EXPRESSION ; MECHANISM ; SURVIVAL ; ISOFORM ; FISHES ; WATER |
WOS类目 | Biology ; Physiology |
WOS研究方向 | Life Sciences & Biomedicine - Other Topics ; Physiology |
来源机构 | 新疆大学 |
资源类型 | 期刊论文 |
条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/167687 |
作者单位 | Xinjiang Univ, Coll Life Sci & Technol, Xinjiang Key Lab Biol Resources & Genet Engn, Urumqi, Peoples R China |
推荐引用方式 GB/T 7714 | Jiang, Min,Ma, Ji,Qiu, Liming. CRYOPROTECTIVE EFFECT OF AN INSECT ANTIFREEZE PROTEIN MPAFP 698 AND ITS MUTANTS FROM THE DESERT BEETLE MICRODERA PUNCTIPENNIS[J]. 新疆大学,2011,32(5):436-446. |
APA | Jiang, Min,Ma, Ji,&Qiu, Liming.(2011).CRYOPROTECTIVE EFFECT OF AN INSECT ANTIFREEZE PROTEIN MPAFP 698 AND ITS MUTANTS FROM THE DESERT BEETLE MICRODERA PUNCTIPENNIS.CRYOLETTERS,32(5),436-446. |
MLA | Jiang, Min,et al."CRYOPROTECTIVE EFFECT OF AN INSECT ANTIFREEZE PROTEIN MPAFP 698 AND ITS MUTANTS FROM THE DESERT BEETLE MICRODERA PUNCTIPENNIS".CRYOLETTERS 32.5(2011):436-446. |
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