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DOI	10.1007/s00216-011-4982-7
	The UHPLC-DAD fingerprinting method for analysis of extracellular metabolites of fungi of the genus Geosmithia (Acomycota: Hypocreales)
	Tylova, Tereza 1,2; Kolarik, Miroslav 1,3; Olsovska, Jana 1,4
通讯作者	Olsovska, Jana
来源期刊	ANALYTICAL AND BIOANALYTICAL CHEMISTRY
ISSN	1618-2642
出版年	2011
卷号	400 期号:9 页码:2943-2952
英文摘要	A new simple ultra-high-performance liquid chromatography method with diode array detection (UHPLC-DAD) was developed for chemical fingerprinting analysis of extracellular metabolites in fermentation broth of Geosmithia spp. The SPE method employing Oasis MCX strong cation-exchange mixed-mode polymeric sorbent was chosen for extraction of the metabolites. The analyses were performed on an Acquity UPLC BEH C18 column (100 x 2.1 mm i.d.; particle size, 1.7 mu m; Waters) using a gradient elution program with an aqueous solution of trifluoroacetic acid and acetonitrile as the mobile phase. The applicability of the method was proved by analysis of 38 strains produced by different species and isolated from different sources (hosts). The results revealed the correlation of obtained UHPLC-DAD fingerprints with taxonomical identity.
英文关键词	Ultra-high-performance liquid chromatography Diode array detection Solid-phase extraction Extracellular secondary metabolites Geosmithia spp. Chromatographic fingerprinting
类型	Article
语种	英语
国家	Czech Republic
收录类别	SCI-E
WOS记录号	WOS:000291037800027
WOS关键词	PERFORMANCE LIQUID-CHROMATOGRAPHY ; TANDEM MASS-SPECTROMETRY ; DIODE-ARRAY DETECTION ; QUALITY-CONTROL ; SECONDARY METABOLITES ; ERIGERON-BREVISCAPUS ; GANODERMA-LUCIDUM ; HOST-RANGE ; LC-MS ; DISCRIMINATION
WOS类目	Biochemical Research Methods ; Chemistry, Analytical
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/167012
作者单位	1.Acad Sci Czech Republic, Inst Microbiol, CR-14220 Prague 4, Czech Republic; 2.Charles Univ Prague, Fac Sci, Prague 12840 2, Czech Republic; 3.Charles Univ Prague, Fac Sci, Prague 12801 2, Czech Republic; 4.Res Inst Brewing & Malting, Prague 12044 2, Czech Republic
推荐引用方式 GB/T 7714	Tylova, Tereza,Kolarik, Miroslav,Olsovska, Jana. The UHPLC-DAD fingerprinting method for analysis of extracellular metabolites of fungi of the genus Geosmithia (Acomycota: Hypocreales)[J],2011,400(9):2943-2952.
APA	Tylova, Tereza,Kolarik, Miroslav,&Olsovska, Jana.(2011).The UHPLC-DAD fingerprinting method for analysis of extracellular metabolites of fungi of the genus Geosmithia (Acomycota: Hypocreales).ANALYTICAL AND BIOANALYTICAL CHEMISTRY,400(9),2943-2952.
MLA	Tylova, Tereza,et al."The UHPLC-DAD fingerprinting method for analysis of extracellular metabolites of fungi of the genus Geosmithia (Acomycota: Hypocreales)".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 400.9(2011):2943-2952.