Knowledge Resource Center for Ecological Environment in Arid Area
Genomic DNA isolation from Artemisia species grown in cold desert high altitude of India | |
Kumar, Jitendra1; Mishra, Gyan P.1; Naik, Pradeep K.2; Murkute, Ashutosh A.1; Srivastava, Ravi B.1 | |
通讯作者 | Kumar, Jitendra |
来源期刊 | AFRICAN JOURNAL OF BIOTECHNOLOGY
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ISSN | 1684-5315 |
出版年 | 2011 |
卷号 | 10期号:37页码:7303-7307 |
英文摘要 | Artemisia which produces a large number of secondary metabolites is naturally found in cold desert high altitude environment of India. Secondary metabolites such as alkaloids, flavonoids, phenols, polysaccharides and terpenes represent a significant barrier to the extraction of pure genomic DNA. Thus, in this study, the DNA extraction protocol to extract pure genomic DNA from different Artemisia species was tailored. The protocol was based on the CTAB method with slight modifications. In the study, 1.6 M NaCl, 2% cetyltrimethyl ammonium bromide (CTAB), 3% polyvinylpyrrolidone (PVP) and 0.5% beta-mercaptoethanol was used in the extraction buffer. The incubation period was kept for 1 h at 65 degrees C with one-tenth of the volume of warm (55 degrees C) 10% CTAB solution during the extraction process. This study described a reliable protocol for extracting good quality and optimum amount of DNA from Artemisia species suitable for PCR analysis. |
英文关键词 | Artemisia genomic DNA isolation PCR amplification secondary metabolites |
类型 | Article |
语种 | 英语 |
国家 | India |
收录类别 | SCI-E |
WOS记录号 | WOS:000294673800025 |
WOS类目 | Biotechnology & Applied Microbiology |
WOS研究方向 | Biotechnology & Applied Microbiology |
资源类型 | 期刊论文 |
条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/166855 |
作者单位 | 1.Def Res Dev Org, Def Inst High Altitude Res, Leh Ladakh 194101, India; 2.Jaypee Univ Informat Technol, Solan 173215, Himachal Prades, India |
推荐引用方式 GB/T 7714 | Kumar, Jitendra,Mishra, Gyan P.,Naik, Pradeep K.,et al. Genomic DNA isolation from Artemisia species grown in cold desert high altitude of India[J],2011,10(37):7303-7307. |
APA | Kumar, Jitendra,Mishra, Gyan P.,Naik, Pradeep K.,Murkute, Ashutosh A.,&Srivastava, Ravi B..(2011).Genomic DNA isolation from Artemisia species grown in cold desert high altitude of India.AFRICAN JOURNAL OF BIOTECHNOLOGY,10(37),7303-7307. |
MLA | Kumar, Jitendra,et al."Genomic DNA isolation from Artemisia species grown in cold desert high altitude of India".AFRICAN JOURNAL OF BIOTECHNOLOGY 10.37(2011):7303-7307. |
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