Arid
Maize breeding for marginal lands: Physiological and molecular approach to decipher response and selection of maize recombinant inbred lines (RILs) under water deficit at early growth stage
Shuja, Malik Nawaz1; Ali, Wajid1; Iqbal, Aqib1; Ali, Ijaz1; Munir, Iqbal1; Ahmad, Dawood1; Inamullah2; Shaheenshah2; Ahmad, Gulzar3; Khan, M. Arif2; Swati, Zahoor Ahmad1
通讯作者Iqbal, Aqib
来源期刊AFRICAN JOURNAL OF BIOTECHNOLOGY
ISSN1684-5315
出版年2011
卷号10期号:18页码:3521-3527
英文摘要

Drought is the major abiotic stress constraining the production of maize (Zea mays L.) in the arid and semi-arid areas. Recombinant inbred lines (RILs) with improved performance from the 6th selection cycles were evaluated against drought stress at early growth stages and genetic distance was determined through random amplified polymorphic DNA (RAPD) primers. Significant variation was observed in the rate of water loss (RWL) at 30, 90 and 150 min after excision, relative water content (RWC) and membrane stability among the RILs. In comparison with check variety Azam, 7 RILs maintained a low RWL, 2 had higher RWC and 6 RILs had higher membrane stability, thus showing a higher degree of adaptation to drought stress. The preliminary results showed polymorphism among the drought sensitive and tolerant RILs. The RILs were clustered into three groups on the basis of amplification pattern obtained with 60 RAPD markers. Variation in the genetic makeup of the tolerant RILs was evident from their clustering in different groups, though most were clustered in group III. Furthermore, one RAPD marker could identify maize genotypes, maintaining low RWL from the excised leaves. We are in the process of sequencing the amplified product of this primer and transforming it into sequence characterized amplified region (SCAR) markers for a more reliable marker assisted selection.


英文关键词Maize recombinant inbred lines (RILs) drought physiological markers random amplified polymorphic DNA (RAPD)
类型Article
语种英语
国家Pakistan
收录类别SCI-E
WOS记录号WOS:000290667500003
WOS关键词MARKER-ASSISTED SELECTION ; QUANTITATIVE TRAIT LOCI ; DROUGHT TOLERANCE ; CROP IMPROVEMENT ; GENOTYPES ; PLANT ; WHEAT ; IDENTIFICATION ; AGRICULTURE ; VARIABILITY
WOS类目Biotechnology & Applied Microbiology
WOS研究方向Biotechnology & Applied Microbiology
资源类型期刊论文
条目标识符http://119.78.100.177/qdio/handle/2XILL650/166845
作者单位1.Univ Agr, Inst Biotechnol & Genet Engn, Mol Genet Lab, Peshawar, Khyber Pakhtoon, Pakistan;
2.Univ Agr, Dept Agron, Peshawar, Khyber Pakhtoon, Pakistan;
3.Cereals Crops Res Inst, Pirsabak, Nowshera, Pakistan
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Shuja, Malik Nawaz,Ali, Wajid,Iqbal, Aqib,et al. Maize breeding for marginal lands: Physiological and molecular approach to decipher response and selection of maize recombinant inbred lines (RILs) under water deficit at early growth stage[J],2011,10(18):3521-3527.
APA Shuja, Malik Nawaz.,Ali, Wajid.,Iqbal, Aqib.,Ali, Ijaz.,Munir, Iqbal.,...&Swati, Zahoor Ahmad.(2011).Maize breeding for marginal lands: Physiological and molecular approach to decipher response and selection of maize recombinant inbred lines (RILs) under water deficit at early growth stage.AFRICAN JOURNAL OF BIOTECHNOLOGY,10(18),3521-3527.
MLA Shuja, Malik Nawaz,et al."Maize breeding for marginal lands: Physiological and molecular approach to decipher response and selection of maize recombinant inbred lines (RILs) under water deficit at early growth stage".AFRICAN JOURNAL OF BIOTECHNOLOGY 10.18(2011):3521-3527.
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