Knowledge Resource Center for Ecological Environment in Arid Area
Maize breeding for marginal lands: Physiological and molecular approach to decipher response and selection of maize recombinant inbred lines (RILs) under water deficit at early growth stage | |
Shuja, Malik Nawaz1; Ali, Wajid1; Iqbal, Aqib1; Ali, Ijaz1; Munir, Iqbal1; Ahmad, Dawood1; Inamullah2; Shaheenshah2; Ahmad, Gulzar3; Khan, M. Arif2; Swati, Zahoor Ahmad1 | |
通讯作者 | Iqbal, Aqib |
来源期刊 | AFRICAN JOURNAL OF BIOTECHNOLOGY
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ISSN | 1684-5315 |
出版年 | 2011 |
卷号 | 10期号:18页码:3521-3527 |
英文摘要 | Drought is the major abiotic stress constraining the production of maize (Zea mays L.) in the arid and semi-arid areas. Recombinant inbred lines (RILs) with improved performance from the 6th selection cycles were evaluated against drought stress at early growth stages and genetic distance was determined through random amplified polymorphic DNA (RAPD) primers. Significant variation was observed in the rate of water loss (RWL) at 30, 90 and 150 min after excision, relative water content (RWC) and membrane stability among the RILs. In comparison with check variety Azam, 7 RILs maintained a low RWL, 2 had higher RWC and 6 RILs had higher membrane stability, thus showing a higher degree of adaptation to drought stress. The preliminary results showed polymorphism among the drought sensitive and tolerant RILs. The RILs were clustered into three groups on the basis of amplification pattern obtained with 60 RAPD markers. Variation in the genetic makeup of the tolerant RILs was evident from their clustering in different groups, though most were clustered in group III. Furthermore, one RAPD marker could identify maize genotypes, maintaining low RWL from the excised leaves. We are in the process of sequencing the amplified product of this primer and transforming it into sequence characterized amplified region (SCAR) markers for a more reliable marker assisted selection. |
英文关键词 | Maize recombinant inbred lines (RILs) drought physiological markers random amplified polymorphic DNA (RAPD) |
类型 | Article |
语种 | 英语 |
国家 | Pakistan |
收录类别 | SCI-E |
WOS记录号 | WOS:000290667500003 |
WOS关键词 | MARKER-ASSISTED SELECTION ; QUANTITATIVE TRAIT LOCI ; DROUGHT TOLERANCE ; CROP IMPROVEMENT ; GENOTYPES ; PLANT ; WHEAT ; IDENTIFICATION ; AGRICULTURE ; VARIABILITY |
WOS类目 | Biotechnology & Applied Microbiology |
WOS研究方向 | Biotechnology & Applied Microbiology |
资源类型 | 期刊论文 |
条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/166845 |
作者单位 | 1.Univ Agr, Inst Biotechnol & Genet Engn, Mol Genet Lab, Peshawar, Khyber Pakhtoon, Pakistan; 2.Univ Agr, Dept Agron, Peshawar, Khyber Pakhtoon, Pakistan; 3.Cereals Crops Res Inst, Pirsabak, Nowshera, Pakistan |
推荐引用方式 GB/T 7714 | Shuja, Malik Nawaz,Ali, Wajid,Iqbal, Aqib,et al. Maize breeding for marginal lands: Physiological and molecular approach to decipher response and selection of maize recombinant inbred lines (RILs) under water deficit at early growth stage[J],2011,10(18):3521-3527. |
APA | Shuja, Malik Nawaz.,Ali, Wajid.,Iqbal, Aqib.,Ali, Ijaz.,Munir, Iqbal.,...&Swati, Zahoor Ahmad.(2011).Maize breeding for marginal lands: Physiological and molecular approach to decipher response and selection of maize recombinant inbred lines (RILs) under water deficit at early growth stage.AFRICAN JOURNAL OF BIOTECHNOLOGY,10(18),3521-3527. |
MLA | Shuja, Malik Nawaz,et al."Maize breeding for marginal lands: Physiological and molecular approach to decipher response and selection of maize recombinant inbred lines (RILs) under water deficit at early growth stage".AFRICAN JOURNAL OF BIOTECHNOLOGY 10.18(2011):3521-3527. |
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