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With increasing food demand worldwide, agriculture in semiarid and and regions becomes increasingly important, though knowledge about organic matter (OM) conserving management systems is scarce. This study aimed at examining organic C (OC) and nitrogen (N) concentrations in various soil OM pools affected by 26-years application of chemical fertilizer and farmyard manure at an and site of Gansu Province, China. Macro OM (> 0.05 mm) was extracted by wet sieving and then separated into light macro OM (< 1.8 g cm(-3)) and heavy macro OM (> 1.8 g cm(-3)) sub-fractions; bulk soil was differentiated into free particulate OM (FPOM, < 1.6 g cm(-3)), occluded particulate OM (OPOM, 1.6-1.8 g cm(-3)) and mineral-associated OM (> 1.8 g cm(-3)). OC and N concentrations of heavy macro OM and FPOM were slightly affected by long-term N fertilization alone and its combination with P and K, but their magnitudes of change had not significantly contributed to total soil OC and N concentrations. Farmyard manure increased light macro OC and N by 58 and 70%, heavy macro OC and N by 86 and 117%, free particulate OC and N by 29 and 55%, occluded particulate OC and N by 29 and 55%,and mineral-associated OC and N by 44 and 48%, respectively, compared to nil-manure. Mineral fertilization improved soil OM quality by decreasing C/N ratio in the light macro OM and FPOM fractions where farmyard manure was absent. Organic manure led to a decline of the C/N ratio in all physically-separated OM fractions possibly due to the increased input of processed organic materials. We found about two thirds of macro OM was actually located within 2-0.05 mm organo-mineral associations or/and aggregates. In conclusion, this study stresses the vital importance to apply organic manure to the wheat-corn production system characterized by straw removal and conventional tillage in the region. (C) 2009 Elsevier Ltd. All rights reserved.