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This work is an evaluation of the quality of three introduced European olive cultivars Arbequitia, Coratina, and Koroneiki grown under a biological agricultural system in the and zone of Sfax in Tunisia compared to Chemlali cultivars. Various olive parameters were analyzed, such as ripening index, pulp/stone (P/S) ratio and oil content. We have considered the regulated physicochemical analytical parameters, fatty acids and minor compounds for olive oil. The most of the quality indices and fatty acid composition showed significant variations among olive cultivars. The Koroneiki, Coratina and Chemiali Zarzis cultivars had highest values of oleic acid (62.7%) (76.3%, 75.8% and 73.9%, respectively). Arbequina, had lowest value of oleic acid and similar to Chemlali Sfax. Coratina was noteworthy for its higher content of phenolic compounds (287.8 mg kg(-1)), oil content (42.4%) and P/S ratio (4.7%). Although their low phenol contents, autochthonous cultivars presented high contents of alpha-tocopherol (577.8 and 434.6 mg kg(-1) for Chemlali Boughrara and Chemiali Zarzis, respectively) except for Chemlali Sfax. In conclusion, European cultivars seem to have adapted to the area studied according to their oil biochemical composition while the increase observed in their alpha-tocopherol levels. The cultivar Arbequina showed a similar composition of Chemlali Sfax. (C) 2009 Elsevier B.V. All rights reserved.