Arid
DOI10.1371/journal.pone.0015633
De Novo Analysis of Transcriptome Dynamics in the Migratory Locust during the Development of Phase Traits
Chen, Shuang; Yang, Pengcheng; Jiang, Feng; Wei, Yuanyuan; Ma, Zongyuan; Kang, Le
通讯作者Chen, Shuang
来源期刊PLOS ONE
ISSN1932-6203
出版年2010
卷号5期号:12
英文摘要

Locusts exhibit remarkable density-dependent phenotype (phase) changes from the solitary to the gregarious, making them one of the most destructive agricultural pests. This phenotype polyphenism arises from a single genome and diverse transcriptomes in different conditions. Here we report a de novo transcriptome for the migratory locust and a comprehensive, representative core gene set. We carried out assembly of 21.5 Gb Illumina reads, generated 72,977 transcripts with N50 2,275 bp and identified 11,490 locust protein-coding genes. Comparative genomics analysis with eight other sequenced insects was carried out to indentify the genomic divergence between hemimetabolous and holometabolous insects for the first time and 18 genes relevant to development was found. We further utilized the quantitative feature of RNA-seq to measure and compare gene expression among libraries. We first discovered how divergence in gene expression between two phases progresses as locusts develop and identified 242 transcripts as candidates for phase marker genes. Together with the detailed analysis of deep sequencing data of the 4(th) instar, we discovered a phase-dependent divergence of biological investment in the molecular level. Solitary locusts have higher activity in biosynthetic pathways while gregarious locusts show higher activity in environmental interaction, in which genes and pathways associated with regulation of neurotransmitter activities, such as neurotransmitter receptors, synthetase, transporters, and GPCR signaling pathways, are strongly involved. Our study, as the largest de novo transcriptome to date, with optimization of sequencing and assembly strategy, can further facilitate the application of de novo transcriptome. The locust transcriptome enriches genetic resources for hemimetabolous insects and our understanding of the origin of insect metamorphosis. Most importantly, we identified genes and pathways that might be involved in locust development and phase change, and may thus benefit pest management.


类型Article
语种英语
国家Peoples R China
收录类别SCI-E
WOS记录号WOS:000285793600023
WOS关键词BEHAVIORALLY RELEVANT ODORS ; CENTRAL-NERVOUS-SYSTEM ; SCHISTOCERCA-GREGARIA ; RNA-SEQ ; ADULT NEUROGENESIS ; DESERT LOCUSTS ; INSTAR LOCUSTS ; FRUIT-FLY ; PROTEIN ; POLYMORPHISM
WOS类目Multidisciplinary Sciences
WOS研究方向Science & Technology - Other Topics
资源类型期刊论文
条目标识符http://119.78.100.177/qdio/handle/2XILL650/166067
作者单位Chinese Acad Sci, Inst Zool, State Key Lab Integrated Management Pest Insects, Beijing, Peoples R China
推荐引用方式
GB/T 7714
Chen, Shuang,Yang, Pengcheng,Jiang, Feng,et al. De Novo Analysis of Transcriptome Dynamics in the Migratory Locust during the Development of Phase Traits[J],2010,5(12).
APA Chen, Shuang,Yang, Pengcheng,Jiang, Feng,Wei, Yuanyuan,Ma, Zongyuan,&Kang, Le.(2010).De Novo Analysis of Transcriptome Dynamics in the Migratory Locust during the Development of Phase Traits.PLOS ONE,5(12).
MLA Chen, Shuang,et al."De Novo Analysis of Transcriptome Dynamics in the Migratory Locust during the Development of Phase Traits".PLOS ONE 5.12(2010).
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