Knowledge Resource Center for Ecological Environment in Arid Area
New expression of bimodal phase distributions in direct-method phasing of protein single-wavelength anomalous diffraction data | |
Zhang Tao1,2,3; Gu Yuan-Xin1,2; Zheng Chao-De1,2; Fan Hai-Fu1,2 | |
通讯作者 | Fan Hai-Fu |
来源期刊 | CHINESE PHYSICS B
![]() |
ISSN | 1674-1056 |
EISSN | 1741-4199 |
出版年 | 2010 |
卷号 | 19期号:8 |
英文摘要 | One of the essential points of the direct-method single-wavelength anomalous diffraction (SAD) phasing for proteins is to express the bimodal SAD phase distribution by the sum of two Gaussian functions peaked respectively at phi(h)’’ + vertical bar Delta phi(h)vertical bar and phi(h)’’ - vertical bar Delta phi(h)vertical bar. The probability for Delta phi(h) being positive (P+) can be derived based on the Cochran distribution in direct methods. Hence the SAD phase ambiguity can be resolved by multiplying the Gaussian function peaked at phi(h)’’ - vertical bar Delta phi(h)vertical bar with P+ and multiplying the Gaussian function peaked at phi(h)’’ - vertical bar Delta phi(h)vertical bar with P- (= 1 - P+). The direct-method SAD phasing has been proved powerful in breaking SAD phase ambiguities, in particular when anomalous-scattering signals are weak. However, the approximation of bimodal phase distributions by the sum of two Gaussian functions introduces considerable errors. In this paper we show that a much better approximation can be achieved by replacing the two Gaussian functions with two von Mises distributions. Test results showed that this leads to significant improvement on the efficiency of direct-method SAD-phasing. |
英文关键词 | direct methods single-wavelength anomalous diffraction OASIS program proteins |
类型 | Article |
语种 | 英语 |
国家 | Peoples R China |
收录类别 | SCI-E |
WOS记录号 | WOS:000280741800002 |
WOS关键词 | ISOMORPHOUS REPLACEMENT ; RESOLUTION ; SCATTERING ; SULFUR |
WOS类目 | Physics, Multidisciplinary |
WOS研究方向 | Physics |
来源机构 | 兰州大学 |
资源类型 | 期刊论文 |
条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/163663 |
作者单位 | 1.Chinese Acad Sci, Beijing Natl Lab Condensed Matter Phys, Inst Phys, Beijing 100190, Peoples R China; 2.Chinese Acad Sci, Key Lab Soft Matter Phys, Inst Phys, Beijing 100190, Peoples R China; 3.Lanzhou Univ, Res Inst Magnet Mat, Sch Phys Sci & Technol, Lanzhou 730000, Peoples R China |
推荐引用方式 GB/T 7714 | Zhang Tao,Gu Yuan-Xin,Zheng Chao-De,et al. New expression of bimodal phase distributions in direct-method phasing of protein single-wavelength anomalous diffraction data[J]. 兰州大学,2010,19(8). |
APA | Zhang Tao,Gu Yuan-Xin,Zheng Chao-De,&Fan Hai-Fu.(2010).New expression of bimodal phase distributions in direct-method phasing of protein single-wavelength anomalous diffraction data.CHINESE PHYSICS B,19(8). |
MLA | Zhang Tao,et al."New expression of bimodal phase distributions in direct-method phasing of protein single-wavelength anomalous diffraction data".CHINESE PHYSICS B 19.8(2010). |
条目包含的文件 | 条目无相关文件。 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。