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DOI10.1002/bmc.1364
High-performance liquid chromatography with solid-phase extraction for the quantitative determination of nilotinib in human plasma
Miura, Masatomo1; Takahashi, Naoto2; Sawada, Ken-ichi2
通讯作者Miura, Masatomo
来源期刊BIOMEDICAL CHROMATOGRAPHY
ISSN0269-3879
出版年2010
卷号24期号:7页码:789-793
英文摘要

A simple, rapid and sensitive high-performance liquid chromatography (HPLC)-based method with ultraviolet detection was developed for the quantitation of nilotinib, a tyrosine kinase inhibitor, in human plasma. Nilotinib and the internal standard dasatinib were separated using a mobile phase of 0.5% KH(2)PO(4)(pH2.5)-acetonitrile-methanol (55:25:20, v/v/v) on a Capcell Pak MG II column (250 x 4.6 mm) at a flow rate of 0.5 mL/min and optical measurement at 250 nm. Analysis required only 100 mu L of plasma and involved a rapid and simple solid-phase extraction with an Oasis HLB cartridge, which gave recoveries from 72 to 78% for nilotinib and from 74 to 76% for dasatinib. The lower limit of quantification for nilotinib was 10 ng/mL. The linear range of this assay was between 10 and 5000 ng/mL (r(2) > 0.9992 for the regression line). Intra- and inter-day coefficients of variation were less than 10.0% and accuracies were within 10.4% over the linear range. Our results indicate that this method is applicable to the monitoring of plasma levels of nilotinib in a clinical setting. Copyright 0 2009 John Wiley & Sons, Ltd.


英文关键词nilotinib dasatinib HPLC human plasma
类型Article
语种英语
国家Japan
收录类别SCI-E
WOS记录号WOS:000279367900016
WOS关键词CHRONIC MYELOID-LEUKEMIA ; BCR-ABL ; INHIBITOR ; IMATINIB ; AMN107
WOS类目Biochemical Research Methods ; Biochemistry & Molecular Biology ; Chemistry, Analytical ; Pharmacology & Pharmacy
WOS研究方向Biochemistry & Molecular Biology ; Chemistry ; Pharmacology & Pharmacy
资源类型期刊论文
条目标识符http://119.78.100.177/qdio/handle/2XILL650/163497
作者单位1.Akita Univ Hosp, Dept Pharm, Akita 0108543, Japan;
2.Akita Univ, Dept Hematol Nephrol & Rheumatol, Grad Sch Med, Akita 010, Japan
推荐引用方式
GB/T 7714
Miura, Masatomo,Takahashi, Naoto,Sawada, Ken-ichi. High-performance liquid chromatography with solid-phase extraction for the quantitative determination of nilotinib in human plasma[J],2010,24(7):789-793.
APA Miura, Masatomo,Takahashi, Naoto,&Sawada, Ken-ichi.(2010).High-performance liquid chromatography with solid-phase extraction for the quantitative determination of nilotinib in human plasma.BIOMEDICAL CHROMATOGRAPHY,24(7),789-793.
MLA Miura, Masatomo,et al."High-performance liquid chromatography with solid-phase extraction for the quantitative determination of nilotinib in human plasma".BIOMEDICAL CHROMATOGRAPHY 24.7(2010):789-793.
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