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High Performance Liquid Chromatographic and Biological Studies of 2-[(4-Methyl-2-nitrophenyl)azo]-3-oxo-N-phenylbutanamide (Yellow Pigment) | |
Yenidogan, Semiha1; Oktav, Mehmet1; Ulgen, Mert2 | |
通讯作者 | Yenidogan, Semiha |
来源期刊 | ASIAN JOURNAL OF CHEMISTRY
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ISSN | 0970-7077 |
出版年 | 2010 |
卷号 | 22期号:5页码:3705-3710 |
英文摘要 | A simple reverse phase high performance liquid chromatographic (HPLC) method was developed for the quantitative determination of 4-methyl-2-nitroaniline (MNA) a potential reduction product of 2-[(4-methyl-2-nitrophenyl)azo]-3-oxo-N-phenylbutanamide (pigment yellow 1, PYI). Quantifications were carried out by Integrations of the peak areas using internal standardization method with N-(2.4-dichlorobenzoyl)aniline (DCBZA) as an internal standard The proposed dye (PY1) and its potential reduction product. 4-methyl-2-nitroaniline (MNA) arid the internal standard were separated by a reverse phase El PLC system The dye was then incubated with NADH to Investigate its possible reduction into the corresponding primary amine (MNA). However, no reduction was observed. The proposed compound was also incubated with rat microsomal preparations fortified with NADPH In both experiments, compounds in the incubates were extracted into dichloromethane (DCM) and finally evaporated under nitrogene. No metabolic reduction to MNA was observed as well. This indicates the in vitro stability of PYI and hence safety of the dye in tuns of potential toxicity from this reduction produet. |
英文关键词 | Azo dyes In vitro metabolism Printing inks Am reduction 2-[(4-Methyl-2-nitrophenyl)azo]-3-oxo-N-phenylbutanamide HPLC |
类型 | Article |
语种 | 英语 |
国家 | Turkey |
收录类别 | SCI-E |
WOS记录号 | WOS:000279844500052 |
WOS关键词 | AZO DYES ; REDUCTION ; PRODUCTS |
WOS类目 | Chemistry, Multidisciplinary |
WOS研究方向 | Chemistry |
资源类型 | 期刊论文 |
条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/163301 |
作者单位 | 1.Marmara Univ Tech, Dept Printing, Fac Educ, TR-34722 Istanbul, Turkey; 2.Acibadem Univ, Sch Associated Hlth Sci, TR-34848 Istanbul, Turkey |
推荐引用方式 GB/T 7714 | Yenidogan, Semiha,Oktav, Mehmet,Ulgen, Mert. High Performance Liquid Chromatographic and Biological Studies of 2-[(4-Methyl-2-nitrophenyl)azo]-3-oxo-N-phenylbutanamide (Yellow Pigment)[J],2010,22(5):3705-3710. |
APA | Yenidogan, Semiha,Oktav, Mehmet,&Ulgen, Mert.(2010).High Performance Liquid Chromatographic and Biological Studies of 2-[(4-Methyl-2-nitrophenyl)azo]-3-oxo-N-phenylbutanamide (Yellow Pigment).ASIAN JOURNAL OF CHEMISTRY,22(5),3705-3710. |
MLA | Yenidogan, Semiha,et al."High Performance Liquid Chromatographic and Biological Studies of 2-[(4-Methyl-2-nitrophenyl)azo]-3-oxo-N-phenylbutanamide (Yellow Pigment)".ASIAN JOURNAL OF CHEMISTRY 22.5(2010):3705-3710. |
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