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With the current water shortage in East Africa improving crop water use is vital especially in the arid and semi-arid regions of Ethiopia. To understand the response of barley to water and to simulate the biomass and grain yield of barley under various water inputs and planting dates, we tested the FAO AquaCrop model versions 3.0 using independent data sets during the cropping seasons of 2006, 2008 and 2009 at Mekelle site in northern Ethiopia. We found that the model is valid to simulate the barley biomass and grain yield under various planting dates in the study site. AquaCrop model can be used in the evaluation of optimal planting time. Out of the tested planting dates, planting on July 4 (early sowing) was found to maximize barley biomass, grain and water use efficiency. The model can also be used in the evaluation of irrigation strategies. Barley showed slightly lower performance under mild water stress condition compared to full irrigation condition. However, the model has indicated the possibility of obtaining more biomass and grain yield from a relatively larger barley field under (deficit irrigation) mild stress condition. (C) 2010 Elsevier BM. All rights reserved.