Arid
DOI10.1007/s11274-008-9891-3
Production of a novel glycerol-inducible lipase from thermophilic Geobacillus stearothermophilus strain-5
Berekaa, Mahmoud M.1; Zaghloul, Taha I.2; Abdel-Fattah, Yasser R.3; Saeed, Hesham M.2; Sifour, Mohamed4
通讯作者Berekaa, Mahmoud M.
来源期刊WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
ISSN0959-3993
出版年2009
卷号25期号:2页码:287-294
英文摘要

In a screening program for isolation of thermophilic lipase-producing bacteria, a number of thermophilic bacteria were isolated from desert soil from Baltim, Egypt. Among 55 isolates, a potent bacterial candidate (starin-5) was characterized and identified by biochemical and PCR techniques, based on 16S rRNA sequencing. Phylogenetic analysis revealed its closeness to geobacilli especially the thermophilic Geobacillus stearothermophilus with optimal growth and lipolytic enzyme activity at 60A degrees C and pH 7.0. An inducible nature of lipolytic enzyme synthesis using glycerol and glucose was demonstrated. Approximately, 94-100% of the original activity was retained due to thermal stability of the crude enzyme after heat treatment for 15 min at 30-60A degrees C. The enzyme retained 84.84% of its original activity during incubation at 70A degrees C (pH 8.0) for 15 min. Lipase enzyme from G. stearothermophilus strain-5 was immobilized on various carriers and the most suitable carrier was chitin that showed 73.03% of activity yield.


英文关键词Geobacillus stearothermophilus Immobilization Production of lipase Thermostable lipase
类型Article
语种英语
国家Egypt ; Algeria
收录类别SCI-E
WOS记录号WOS:000263676600014
WOS关键词THERMOSTABLE ALKALINE LIPASE ; BACILLUS SP ; LIPOLYTIC ENZYME ; PURIFICATION ; IMMOBILIZATION ; OPTIMIZATION ; SUBTILIS ; DESIGN
WOS类目Biotechnology & Applied Microbiology
WOS研究方向Biotechnology & Applied Microbiology
资源类型期刊论文
条目标识符http://119.78.100.177/qdio/handle/2XILL650/162835
作者单位1.Univ Alexandria, Fac Sci Moharam Bay, Dept Environm Sci, Alexandria, Egypt;
2.Univ Alexandria, Inst Grad Studies & Res, Dept Biotechnol, Alexandria, Egypt;
3.Mubarak City Sci Res & Technol Applicat, Alexandria, Egypt;
4.Univ Jijel, Fac Sci, Dept Mol & Cell Biol, Jijel, Algeria
推荐引用方式
GB/T 7714
Berekaa, Mahmoud M.,Zaghloul, Taha I.,Abdel-Fattah, Yasser R.,et al. Production of a novel glycerol-inducible lipase from thermophilic Geobacillus stearothermophilus strain-5[J],2009,25(2):287-294.
APA Berekaa, Mahmoud M.,Zaghloul, Taha I.,Abdel-Fattah, Yasser R.,Saeed, Hesham M.,&Sifour, Mohamed.(2009).Production of a novel glycerol-inducible lipase from thermophilic Geobacillus stearothermophilus strain-5.WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,25(2),287-294.
MLA Berekaa, Mahmoud M.,et al."Production of a novel glycerol-inducible lipase from thermophilic Geobacillus stearothermophilus strain-5".WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY 25.2(2009):287-294.
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