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DOI | 10.1007/s11274-008-9891-3 |
Production of a novel glycerol-inducible lipase from thermophilic Geobacillus stearothermophilus strain-5 | |
Berekaa, Mahmoud M.1; Zaghloul, Taha I.2; Abdel-Fattah, Yasser R.3; Saeed, Hesham M.2; Sifour, Mohamed4 | |
通讯作者 | Berekaa, Mahmoud M. |
来源期刊 | WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
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ISSN | 0959-3993 |
出版年 | 2009 |
卷号 | 25期号:2页码:287-294 |
英文摘要 | In a screening program for isolation of thermophilic lipase-producing bacteria, a number of thermophilic bacteria were isolated from desert soil from Baltim, Egypt. Among 55 isolates, a potent bacterial candidate (starin-5) was characterized and identified by biochemical and PCR techniques, based on 16S rRNA sequencing. Phylogenetic analysis revealed its closeness to geobacilli especially the thermophilic Geobacillus stearothermophilus with optimal growth and lipolytic enzyme activity at 60A degrees C and pH 7.0. An inducible nature of lipolytic enzyme synthesis using glycerol and glucose was demonstrated. Approximately, 94-100% of the original activity was retained due to thermal stability of the crude enzyme after heat treatment for 15 min at 30-60A degrees C. The enzyme retained 84.84% of its original activity during incubation at 70A degrees C (pH 8.0) for 15 min. Lipase enzyme from G. stearothermophilus strain-5 was immobilized on various carriers and the most suitable carrier was chitin that showed 73.03% of activity yield. |
英文关键词 | Geobacillus stearothermophilus Immobilization Production of lipase Thermostable lipase |
类型 | Article |
语种 | 英语 |
国家 | Egypt ; Algeria |
收录类别 | SCI-E |
WOS记录号 | WOS:000263676600014 |
WOS关键词 | THERMOSTABLE ALKALINE LIPASE ; BACILLUS SP ; LIPOLYTIC ENZYME ; PURIFICATION ; IMMOBILIZATION ; OPTIMIZATION ; SUBTILIS ; DESIGN |
WOS类目 | Biotechnology & Applied Microbiology |
WOS研究方向 | Biotechnology & Applied Microbiology |
资源类型 | 期刊论文 |
条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/162835 |
作者单位 | 1.Univ Alexandria, Fac Sci Moharam Bay, Dept Environm Sci, Alexandria, Egypt; 2.Univ Alexandria, Inst Grad Studies & Res, Dept Biotechnol, Alexandria, Egypt; 3.Mubarak City Sci Res & Technol Applicat, Alexandria, Egypt; 4.Univ Jijel, Fac Sci, Dept Mol & Cell Biol, Jijel, Algeria |
推荐引用方式 GB/T 7714 | Berekaa, Mahmoud M.,Zaghloul, Taha I.,Abdel-Fattah, Yasser R.,et al. Production of a novel glycerol-inducible lipase from thermophilic Geobacillus stearothermophilus strain-5[J],2009,25(2):287-294. |
APA | Berekaa, Mahmoud M.,Zaghloul, Taha I.,Abdel-Fattah, Yasser R.,Saeed, Hesham M.,&Sifour, Mohamed.(2009).Production of a novel glycerol-inducible lipase from thermophilic Geobacillus stearothermophilus strain-5.WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,25(2),287-294. |
MLA | Berekaa, Mahmoud M.,et al."Production of a novel glycerol-inducible lipase from thermophilic Geobacillus stearothermophilus strain-5".WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY 25.2(2009):287-294. |
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