干旱区生态环境知识资源中心(Arid): Development of a transformation protocol for Tecomella undulata (Smith) Seem from cotyledonary node explants

Arid

DOI	10.1016/j.scienta.2009.01.007
	Development of a transformation protocol for Tecomella undulata (Smith) Seem from cotyledonary node explants
	Aslam, Mohammad; Singh, Rajio; Anandhan, Sivalingam; Pande, Veena; Ahmed, Zakwan
通讯作者	Aslam, Mohammad
来源期刊	SCIENTIA HORTICULTURAE
ISSN	0304-4238
EISSN	1879-1018
出版年	2009
卷号	121 期号:1 页码:119-121
英文摘要	A protocol was optimized for genetic transformation of Rohida (Tecomella undulata) from cotyledonary node tissues using Agrobacterium tumefaciens strain GV2260 harboring binary vector pBinAR containing osmotin and nptII gene under control of CaMV35S promoter. This is the first report on the transformation of T. undulata. The effective concentration of selectable marker antibiotic Used for Screening of transformants was 85.97 mu M in shooting media and 42.98 mu M in rooting media. PCR and Southern blot confirmed integration of osmotin gene into genome of Rohida. (C) 2009 Elsevier B.V. All rights reserved.
英文关键词	Agrobacterium tumefaciens Tecomella undulata Cotyledonary node
类型	Article
语种	英语
国家	India
收录类别	SCI-E
WOS记录号	WOS:000266149700020
WOS关键词	TISSUE-CULTURE ; MULTIPLICATION ; ADAPTATION ; DESERT ; CELLS ; TREE
WOS类目	Horticulture
WOS研究方向	Agriculture
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/162556
作者单位	Def Inst Bioenergy Res, Naini Tal 263139, Uttarakhand, India
推荐引用方式 GB/T 7714	Aslam, Mohammad,Singh, Rajio,Anandhan, Sivalingam,et al. Development of a transformation protocol for Tecomella undulata (Smith) Seem from cotyledonary node explants[J],2009,121(1):119-121.
APA	Aslam, Mohammad,Singh, Rajio,Anandhan, Sivalingam,Pande, Veena,&Ahmed, Zakwan.(2009).Development of a transformation protocol for Tecomella undulata (Smith) Seem from cotyledonary node explants.SCIENTIA HORTICULTURAE,121(1),119-121.
MLA	Aslam, Mohammad,et al."Development of a transformation protocol for Tecomella undulata (Smith) Seem from cotyledonary node explants".SCIENTIA HORTICULTURAE 121.1(2009):119-121.