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This study provides a comprehensive spatio-temporal assessment of the surface water resources of the semi-arid Karkheh basin, Iran, and consequently enables decision makers to work towards a sustainable water development in that region. The analysis is based on the examination of statistical parameters, flow duration characteristics, base flow separation and trend analysis for which data of seven key gauging stations were used for the period of 1961-2001. Additionally, basin level water accounting was carried out for the water year 1993-94. The study shows that observed daily, monthly and annual streamflows are highly variable in space and time within the basin. The streamflows have not been changed significantly at annual scale, but few months have shown significant trends, most notably a decline during May and June and an increase during December and March. The major causes were related to changes in climate, land use and reservoir operations. The study concludes that the water allocations to different sectors were lower than the totally available resources during the study period. However, looking at the high variability of streamflows, changes in climate and land use and ongoing water resources development planning, it will be extremely difficult to meet the demands of all sectors in the future, particularly during dry years. (C) 2008 Elsevier Ltd. All rights reserved.