Knowledge Resource Center for Ecological Environment in Arid Area
DOI | 10.1021/jf802557m |
Insecticidal Action of PF2 Lectin from Olneya tesota (Palo Fierro) against Zabrotes subfasciatus Larvae and Midgut Glycoconjugate Binding | |
Lagarda-Diaz, Irlanda1; Guzman-Partida, Ana M.1; Urbano-Hernandez, Gabriel1; Magdalena Ortega-Neblas, M.2; Refugio Robles-Burgueno, M.1; Winzerling, Joy3; Vazquez-Moreno, Luz1 | |
通讯作者 | Vazquez-Moreno, Luz |
来源期刊 | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
![]() |
ISSN | 0021-8561 |
EISSN | 1520-5118 |
出版年 | 2009 |
卷号 | 57期号:2页码:689-694 |
英文摘要 | Zabrotes subfasciatus (Boheman) is the main pest of common beans (Phaselous vulgaris). Some wild legume seeds may contain lectins with insecticidal activities against this insect. The larval developments of Z. subfasciatus on seeds of Olneya tesota (a desert wild legume) and on artificial seeds containing purified PF2 lectin were evaluated. PF2 susceptibility to proteolysis was assessed by incubation with midgut extract at different times. PF2 binding to midgut glycoconjugates was assessed by histochemistry. A reduced level of oviposition and a lack of emergence of adult beetles were observed in O. tesota seeds (compared to common beans), and in artificial seeds containing PF2 at 0.5 and 1%. PF2 was resistant to larval midgut proteolysis for 24 h, while PHA-E (lectin control) was fully digested after 4 h. Histochemistry analysis of midguts incubated with PF2 showed recognition for microvillae and possibly with peritrophic gel. On the other hand, PHA-E exhibited no interaction with larval midgut glycoproteins. Proteolysis resistance and glycan recognition could in part explain why PF2 is toxic to Z. subfasciatus while PHA is not. |
英文关键词 | Insecticidal lectins wild legume Zabrotes subfasciatus insect midgut |
类型 | Article |
语种 | 英语 |
国家 | Mexico ; USA |
收录类别 | SCI-E |
WOS记录号 | WOS:000262665000053 |
WOS关键词 | CALLOSOBRUCHUS-MACULATUS COLEOPTERA ; TALISIA-ESCULENTA LECTIN ; SNOWDROP LECTIN ; PERITROPHIC MEMBRANE ; NILAPARVATA-LUGENS ; PHASEOLUS-VULGARIS ; PLANT-LECTINS ; BEAN WEEVIL ; LEAF LECTIN ; RESISTANCE |
WOS类目 | Agriculture, Multidisciplinary ; Chemistry, Applied ; Food Science & Technology |
WOS研究方向 | Agriculture ; Chemistry ; Food Science & Technology |
来源机构 | University of Arizona |
资源类型 | 期刊论文 |
条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/161191 |
作者单位 | 1.Ctr Invest Alimentac & Desarrollo, Hermosillo, Sonora, Mexico; 2.Univ Sonora, Dept Invest Cient & Tecnol, Hermosillo 83000, Sonora, Mexico; 3.Univ Arizona, Dept Nutr Sci, Tucson, AZ 85721 USA |
推荐引用方式 GB/T 7714 | Lagarda-Diaz, Irlanda,Guzman-Partida, Ana M.,Urbano-Hernandez, Gabriel,et al. Insecticidal Action of PF2 Lectin from Olneya tesota (Palo Fierro) against Zabrotes subfasciatus Larvae and Midgut Glycoconjugate Binding[J]. University of Arizona,2009,57(2):689-694. |
APA | Lagarda-Diaz, Irlanda.,Guzman-Partida, Ana M..,Urbano-Hernandez, Gabriel.,Magdalena Ortega-Neblas, M..,Refugio Robles-Burgueno, M..,...&Vazquez-Moreno, Luz.(2009).Insecticidal Action of PF2 Lectin from Olneya tesota (Palo Fierro) against Zabrotes subfasciatus Larvae and Midgut Glycoconjugate Binding.JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,57(2),689-694. |
MLA | Lagarda-Diaz, Irlanda,et al."Insecticidal Action of PF2 Lectin from Olneya tesota (Palo Fierro) against Zabrotes subfasciatus Larvae and Midgut Glycoconjugate Binding".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 57.2(2009):689-694. |
条目包含的文件 | 条目无相关文件。 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。