干旱区生态环境知识资源中心(Arid): A scFv Antibody-Based Immunoaffinity Chromatography Column for Clean-Up of Bisphenol A-Contaminated Water Samples

Arid

DOI	10.1021/jf802781t
	A scFv Antibody-Based Immunoaffinity Chromatography Column for Clean-Up of Bisphenol A-Contaminated Water Samples
	Inui, Hideyuki 1; Takehara, Akira 2; Doi, Fumito 2; Nishi, Kosuke 1,2; Takai, Mikio 1,2; Miyake, Shiro 3; Ohkawa, Hideo 1,2
通讯作者	Ohkawa, Hideo
来源期刊	JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
ISSN	0021-8561
EISSN	1520-5118
出版年	2009
卷号	57 期号:2 页码:353-358
英文摘要	This is a report on the development of immunoaffinity chromatography using a column of silica gel with an immobilized single-chain variable fragment (scFv) antibody specific to bisphenol A (BPA) for cleanup of BPA-contaminated water samples. The BBA-2187 scFv antibody specific to BPA was purified from the periplasmic fractions of the recombinant Escherichia coli. After a sample of BPA-contaminated river water was applied to the immunoaffinity column, the background signal intensity observed in high-performance liquid chromatography (HPLC) analysis of the eluates was markedly lower than that observed in HPLC analysis of the eluates from an Oasis HLB cartridge treated with the same sample. The immunoaffinity column efficiently concentrated BPA from actual river water samples with different matrices. Our results demonstrate that the immunoaffinity column with immobilized BBA-2187 scFv antibody is efficient for the cleanup of BPA-contaminated water samples from different sources.
英文关键词	Bisphenol A enzyme-linked Immunosorbent assay immunoaffinity column single-chain variable fragment antibody
类型	Article
语种	英语
国家	Japan
收录类别	SCI-E
WOS记录号	WOS:000262665000006
WOS关键词	PERFORMANCE LIQUID-CHROMATOGRAPHY ; RECOMBINANT FAB ANTIBODIES ; SOLID-PHASE EXTRACTION ; ESCHERICHIA-COLI ; XENOESTROGENS ; EXPRESSION ; FRAGMENTS ; CLONING ; SILICA
WOS类目	Agriculture, Multidisciplinary ; Chemistry, Applied ; Food Science & Technology
WOS研究方向	Agriculture ; Chemistry ; Food Science & Technology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/161190
作者单位	1.Kobe Univ, Res Ctr Environm Genom, Nada Ku, Kobe, Hyogo 6578501, Japan; 2.Fukuyama Univ, Grad Sch Sci & Technol, Hiroshima 7290292, Japan; 3.Horiba Biotechnol Co Ltd, Kyoto 6018315, Japan
推荐引用方式 GB/T 7714	Inui, Hideyuki,Takehara, Akira,Doi, Fumito,et al. A scFv Antibody-Based Immunoaffinity Chromatography Column for Clean-Up of Bisphenol A-Contaminated Water Samples[J],2009,57(2):353-358.
APA	Inui, Hideyuki.,Takehara, Akira.,Doi, Fumito.,Nishi, Kosuke.,Takai, Mikio.,...&Ohkawa, Hideo.(2009).A scFv Antibody-Based Immunoaffinity Chromatography Column for Clean-Up of Bisphenol A-Contaminated Water Samples.JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,57(2),353-358.
MLA	Inui, Hideyuki,et al."A scFv Antibody-Based Immunoaffinity Chromatography Column for Clean-Up of Bisphenol A-Contaminated Water Samples".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 57.2(2009):353-358.