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DOI | 10.1007/s00216-008-2576-9 |
Ultra performance liquid chromatography tandem mass spectrometry performance evaluation for analysis of antibiotics in natural waters | |
Tamtam, Fatima1; Mercier, Fabien2; Eurin, Joelle1; Chevreuil, Marc1; Le Bot, Barbara2 | |
通讯作者 | Tamtam, Fatima |
来源期刊 | ANALYTICAL AND BIOANALYTICAL CHEMISTRY
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ISSN | 1618-2642 |
出版年 | 2009 |
卷号 | 393期号:6-7页码:1709-1718 |
英文摘要 | An ultra performance liquid chromatography electrospray tandem mass spectrometry (UPLC/MS/MS) method was developed and validated for the determination of 17 antibiotics in natural waters in one single extraction and chromatographic procedure. Gradient separation conditions were optimised for 17 compounds belonging to five different antibiotic groups: quinolones (oxolinic acid, nalidixic acid, pipemidic acid, flumequine), fluoroquinolones (enoxacin, ciprofloxacin, norfloxacin, ofloxacin, enrofloxacin, sarafloxacin, danofloxacin, difloxacin, lomefloxacin), sulphonamides (sulphamethoxazole, sulphamethazine), nitroimidazole (ornidazole) and diaminopyrimidine (trimethoprim). The separation of all compounds, obtained using a 1.7 mu m particle size column (100 mm x 2.1 mm), was achieved within 10 min time. Water samples were adjusted to pH 7 and extracted using Oasis hydrophilic-lipophilic balance (HLB) solid phase extraction cartridges. After elution with methanol and concentration, extracts were injected in a C18 column (Acquity UPLC BEH C18) and detected by tandem mass spectrometry. Average recovery from 100 ng L(-1) fortified samples was higher than 70% for most of the compounds, with relative standard deviations below 20%. Performances of the method (recoveries, detection limit, quantification limit and relative standard deviation) and matrix effects were studied, and results obtained showed that method was suitable for routine analysis of antibiotics in surface water. Samples analysis from Seine River (France) confirmed the interest of antibiotic contamination evaluation in that area. |
英文关键词 | UPLC Antibiotics River water Quinolones Sulphonamides Matrix effects Solid phase extraction |
类型 | Article |
语种 | 英语 |
国家 | France |
收录类别 | SCI-E |
WOS记录号 | WOS:000263826100019 |
WOS关键词 | SOLID-PHASE EXTRACTION ; DIFFERENT THERAPEUTIC CLASSES ; SEWAGE-TREATMENT PLANTS ; WASTE-WATER ; SURFACE WATERS ; SULFONAMIDE ANTIMICROBIALS ; BASIC PHARMACEUTICALS ; TRACE DETERMINATION ; QUADRUPOLE-TIME ; WASTEWATERS |
WOS类目 | Biochemical Research Methods ; Chemistry, Analytical |
WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
资源类型 | 期刊论文 |
条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/159685 |
作者单位 | 1.Univ Paris 06, Lab Hydrol & Environm, EPHE, UMR Sisyphe 7619, F-75252 Paris, France; 2.EHESP, LERES, F-35043 Rennes, France |
推荐引用方式 GB/T 7714 | Tamtam, Fatima,Mercier, Fabien,Eurin, Joelle,et al. Ultra performance liquid chromatography tandem mass spectrometry performance evaluation for analysis of antibiotics in natural waters[J],2009,393(6-7):1709-1718. |
APA | Tamtam, Fatima,Mercier, Fabien,Eurin, Joelle,Chevreuil, Marc,&Le Bot, Barbara.(2009).Ultra performance liquid chromatography tandem mass spectrometry performance evaluation for analysis of antibiotics in natural waters.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,393(6-7),1709-1718. |
MLA | Tamtam, Fatima,et al."Ultra performance liquid chromatography tandem mass spectrometry performance evaluation for analysis of antibiotics in natural waters".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 393.6-7(2009):1709-1718. |
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