Knowledge Resource Center for Ecological Environment in Arid Area
DOI | 10.1016/j.aca.2008.12.012 |
Rapid Folin-Ciocalteu method using microtiter 96-well plate cartridges for solid phase extraction to assess urinary total phenolic compounds, as a biomarker of total polyphenols intake | |
Medina-Remon, Alexander1,3,4; Barrionuevo-Gonzalez, Ana1,3,4; Zamora-Ros, Raul1,5; Andres-Lacueva, Cristina1,5; Estruch, Ramon2,3,4; Martinez-Gonzalez, Miguel-Angel3,4,6; Diez-Espino, Javier7; Lamuela-Raventos, Rosa M.1,3,4 | |
通讯作者 | Lamuela-Raventos, Rosa M. |
来源期刊 | ANALYTICA CHIMICA ACTA
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ISSN | 0003-2670 |
EISSN | 1873-4324 |
出版年 | 2009 |
卷号 | 634期号:1页码:54-60 |
英文摘要 | Nutritional markers have several advantages for epidemiologic and clinical assays, when compared to dietary data obtained by food frequency questionnaires. Few studies have assessed whether total polyphenol (TP) compounds provide a valid biomarker for TP intake. To date, there has been almost no literature describing methods to determine TP in complex matrices such as urine, which have many interfering substances. We report a rapid Folin-Ciocalteu method to determine TP in urine samples using Oasis (R) MAX 96-well plate cartridges for solid phase extraction. These plates allow analysis of a high number of samples at the same time. We performed a prospective, randomized, crossover trial and one cross-sectional study with 60 volunteers from the PREDIMED trial, seeking to evaluate whether the TP in urine were correlated with polyphenol intake and Could, therefore, be considered as a market of intake of these compounds. The assay was optimized; the sensitivity and the polarity range of urine polyphenols were increased and the detection and quantification limits were significantly reduced. The metabolites in standards solution and urine samples were stable under the storage and handling conditions. In the clinical trial and the cross-sectional study,TP excreted in Spot urine samples were positively correlated with TP intake, r=0.48, P<0.01 and r=0.257, P=0.04. respectively. The methodology described may be used to detect TP in urine samples, employing the high throughput of 96-well microtiter plates and reader. The method is fast and simple and it allows analysis of a large number of samples at the same time. (C) 2008 Elsevier B.V. All rights reserved. |
英文关键词 | Solid phase extraction 96-Well plate Phenols Folin-Ciocalteu Biomarker Urine PREDIMED Polyphenols stability |
类型 | Article |
语种 | 英语 |
国家 | Spain |
收录类别 | SCI-E |
WOS记录号 | WOS:000263189500008 |
WOS关键词 | DIETARY POLYPHENOLS ; MEDITERRANEAN DIET ; MASS-SPECTROMETRY ; RANDOMIZED-TRIAL ; RISK-FACTORS ; OLIVE OIL ; ASSAY ; EXCRETION ; ACID ; BIOAVAILABILITY |
WOS类目 | Chemistry, Analytical |
WOS研究方向 | Chemistry |
资源类型 | 期刊论文 |
条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/159681 |
作者单位 | 1.Univ Barcelona, Dept Nutr & Food Sci, XaRTA, INSA Pharm Sch, E-08028 Barcelona, Spain; 2.Univ Barcelona, Hosp Clin, Dept Internal Med, IDIBAPS, E-08028 Barcelona, Spain; 3.Inst Salud Carlos III, CIBER Physiopathol Obes & Nutr 06 003 CIBEROBN, Barcelona, Spain; 4.Inst Salud Carlos III, RETICS RD06 0045 0003, Barcelona, Spain; 5.Inst Salud Carlos III, Ingenio CONSOLIDER Program, FUN C FOOD, Barcelona, Spain; 6.Univ Navarra, Clin Univ, Dept Prevent Med & Publ Hlth, E-31080 Pamplona, Spain; 7.Tafalla Hlth Ctr, Osasunbidea, Tafalla, Spain |
推荐引用方式 GB/T 7714 | Medina-Remon, Alexander,Barrionuevo-Gonzalez, Ana,Zamora-Ros, Raul,et al. Rapid Folin-Ciocalteu method using microtiter 96-well plate cartridges for solid phase extraction to assess urinary total phenolic compounds, as a biomarker of total polyphenols intake[J],2009,634(1):54-60. |
APA | Medina-Remon, Alexander.,Barrionuevo-Gonzalez, Ana.,Zamora-Ros, Raul.,Andres-Lacueva, Cristina.,Estruch, Ramon.,...&Lamuela-Raventos, Rosa M..(2009).Rapid Folin-Ciocalteu method using microtiter 96-well plate cartridges for solid phase extraction to assess urinary total phenolic compounds, as a biomarker of total polyphenols intake.ANALYTICA CHIMICA ACTA,634(1),54-60. |
MLA | Medina-Remon, Alexander,et al."Rapid Folin-Ciocalteu method using microtiter 96-well plate cartridges for solid phase extraction to assess urinary total phenolic compounds, as a biomarker of total polyphenols intake".ANALYTICA CHIMICA ACTA 634.1(2009):54-60. |
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