Arid
DOI10.1016/j.aca.2008.12.012
Rapid Folin-Ciocalteu method using microtiter 96-well plate cartridges for solid phase extraction to assess urinary total phenolic compounds, as a biomarker of total polyphenols intake
Medina-Remon, Alexander1,3,4; Barrionuevo-Gonzalez, Ana1,3,4; Zamora-Ros, Raul1,5; Andres-Lacueva, Cristina1,5; Estruch, Ramon2,3,4; Martinez-Gonzalez, Miguel-Angel3,4,6; Diez-Espino, Javier7; Lamuela-Raventos, Rosa M.1,3,4
通讯作者Lamuela-Raventos, Rosa M.
来源期刊ANALYTICA CHIMICA ACTA
ISSN0003-2670
EISSN1873-4324
出版年2009
卷号634期号:1页码:54-60
英文摘要

Nutritional markers have several advantages for epidemiologic and clinical assays, when compared to dietary data obtained by food frequency questionnaires. Few studies have assessed whether total polyphenol (TP) compounds provide a valid biomarker for TP intake. To date, there has been almost no literature describing methods to determine TP in complex matrices such as urine, which have many interfering substances.


We report a rapid Folin-Ciocalteu method to determine TP in urine samples using Oasis (R) MAX 96-well plate cartridges for solid phase extraction. These plates allow analysis of a high number of samples at the same time. We performed a prospective, randomized, crossover trial and one cross-sectional study with 60 volunteers from the PREDIMED trial, seeking to evaluate whether the TP in urine were correlated with polyphenol intake and Could, therefore, be considered as a market of intake of these compounds.


The assay was optimized; the sensitivity and the polarity range of urine polyphenols were increased and the detection and quantification limits were significantly reduced. The metabolites in standards solution and urine samples were stable under the storage and handling conditions. In the clinical trial and the cross-sectional study,TP excreted in Spot urine samples were positively correlated with TP intake, r=0.48, P<0.01 and r=0.257, P=0.04. respectively.


The methodology described may be used to detect TP in urine samples, employing the high throughput of 96-well microtiter plates and reader. The method is fast and simple and it allows analysis of a large number of samples at the same time. (C) 2008 Elsevier B.V. All rights reserved.


英文关键词Solid phase extraction 96-Well plate Phenols Folin-Ciocalteu Biomarker Urine PREDIMED Polyphenols stability
类型Article
语种英语
国家Spain
收录类别SCI-E
WOS记录号WOS:000263189500008
WOS关键词DIETARY POLYPHENOLS ; MEDITERRANEAN DIET ; MASS-SPECTROMETRY ; RANDOMIZED-TRIAL ; RISK-FACTORS ; OLIVE OIL ; ASSAY ; EXCRETION ; ACID ; BIOAVAILABILITY
WOS类目Chemistry, Analytical
WOS研究方向Chemistry
资源类型期刊论文
条目标识符http://119.78.100.177/qdio/handle/2XILL650/159681
作者单位1.Univ Barcelona, Dept Nutr & Food Sci, XaRTA, INSA Pharm Sch, E-08028 Barcelona, Spain;
2.Univ Barcelona, Hosp Clin, Dept Internal Med, IDIBAPS, E-08028 Barcelona, Spain;
3.Inst Salud Carlos III, CIBER Physiopathol Obes & Nutr 06 003 CIBEROBN, Barcelona, Spain;
4.Inst Salud Carlos III, RETICS RD06 0045 0003, Barcelona, Spain;
5.Inst Salud Carlos III, Ingenio CONSOLIDER Program, FUN C FOOD, Barcelona, Spain;
6.Univ Navarra, Clin Univ, Dept Prevent Med & Publ Hlth, E-31080 Pamplona, Spain;
7.Tafalla Hlth Ctr, Osasunbidea, Tafalla, Spain
推荐引用方式
GB/T 7714
Medina-Remon, Alexander,Barrionuevo-Gonzalez, Ana,Zamora-Ros, Raul,et al. Rapid Folin-Ciocalteu method using microtiter 96-well plate cartridges for solid phase extraction to assess urinary total phenolic compounds, as a biomarker of total polyphenols intake[J],2009,634(1):54-60.
APA Medina-Remon, Alexander.,Barrionuevo-Gonzalez, Ana.,Zamora-Ros, Raul.,Andres-Lacueva, Cristina.,Estruch, Ramon.,...&Lamuela-Raventos, Rosa M..(2009).Rapid Folin-Ciocalteu method using microtiter 96-well plate cartridges for solid phase extraction to assess urinary total phenolic compounds, as a biomarker of total polyphenols intake.ANALYTICA CHIMICA ACTA,634(1),54-60.
MLA Medina-Remon, Alexander,et al."Rapid Folin-Ciocalteu method using microtiter 96-well plate cartridges for solid phase extraction to assess urinary total phenolic compounds, as a biomarker of total polyphenols intake".ANALYTICA CHIMICA ACTA 634.1(2009):54-60.
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