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Disease has became an increasingly important issue for wildlife management over the past two decades. Adequate surveillance is fundamental for disease prevention and control thus there is an increasing need for diagnostic assays for wildlife management. The objective of this Study was to evaluate the performance of a field-portable biosensor adapted for rapid detection of specific antibodies in tortoise plasma that reflect a history of exposure to Mycoplasma agassizii which is an agent of tortoise upper respiratory tract disease. Banked plasma samples were tested in two blinded trials. and the parameters that define the reliability of a diagnostic test were estimated based on externally validated tortoise plasma controls. The mean sensitivity of the biosensor (ability to identify exposed tortoises in the group of all exposed individuals) was 78%: the mean specificity (unexposed individuals with negative test result, Out of all unexposed individuals tested) was 73%: the mean positive predictive value (exposed individuals with positive test, Out of all individuals with positive test) was 82%: the mean negative predictive Value (unexposed individuals with negative test. out of all individuals with negative test) was 68%. In a 15-min field-portable format. the biosensor was able to discriminate between true seropositive (n = 34) and true seronegative (n = 231) tortoise plasma with overall accuracy of 84%. The goals established for the tortoise population can help managers decide whether potential diagnostic errors should impact management decision-making, and whether the benefits of the field-portable format of the biosensor assay outweigh any potential disadvantages. 2008 Elsevier B.V. All rights reserved.