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To understand the dynamics of soil organic matter (SOM) in the Eurasian steppe, several soil and meteorological properties were tested in order to estimate the amounts of potentially mineralizable organic carbon (PMC) and nitrogen (PMN). Total 41 surface soil samples were collected in Ukraine and Kazakhstan from cropland, forest, grassland, and desert ecosystems. The fresh soils were incubated for 133 days under constant temperature and moisture conditions, and the CO, emissions and the mineral N from the soils were monitored. PMC and PMN were determined by fitting models to the cumulative curves of the CO2 and the mineral N. Tested soil properties included soil pH, sand, silt and clay contents, carbon and nitrogen contents of light fraction (LF, < 1.6 g cm(-3)) and heavy fraction (HF), and C/N ratio of LF and HF. The meteorological properties considered were mean animal temperature and precipitation. Using multiple regression analysis with the stepwise method, PMC was well estimated by carbon content of LF (LFC) and clay content, compared to the simple correlation with orgarnic carbon (0C). similarly, PMN was better determined by nitrogen content of LF (LFN) and clay content. These results sugggest the partially labile nature of clay-associating OM and of LFC and LFN. The higher PMC and PMN in the forest and grassland sites Would be attributed to the higher LFC and LFN, while the lower LFC and LFN in cropland sites would suggest the relatively higher contribution of clay-associating OM to PMC and PMN. (C) 2007 Elsevier Ltd. All rights reserved.