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The effect of water restriction on milk yield and composition. feed intake, body weight and blood parameters was evaluated in Comisana sheep reared under intensive condition. The experiment, lasted 40 days, was performed on 26 lactating ewes subdivided into three treatment groups: the control T group received water ad libitum (W-100) and Group I (W-80) and Group 2 (W-60) were watered, respectively, with 80 and 60% of the water consumed by the control T group. Water was supplied in the morning once a clay while feed twice. Milk quality analysis was performed every 10 clays for pH, fat, total protein, lactose and somatic cell content. Serum samples, performed ill days 0, 13, 26 and 40. were analyzed for glucose, cholesterol, triglycerides, creatinine, total proteins, albumin. sodium, potassium, calcium and chloride. Compared to W-100. W-60 was significantly different (P<0.05) for body weight and serum potassium levels and showed an increase (P<0.01) in serum concentrations of triglycerides, albumin. total proteins and cholesterol (P<0.05). Total proteins, triglycerides and sodium levels increased (P<0.05) in W-80. Body weight showed a significant difference (P<0.05) only in W-60 if compared to W-100. No significant differences were observed in milk yield, milk composition rind feed intake across the treatment groups. (C) 2008 Published by Elsevier B.V.