Arid
DOI10.1016/j.mimet.2008.07.022
Western blot can distinguish natural and acquired antibodies to Mycoplasma agassizii in the desert tortoise (Gopherus agassizii)
Hunter, Kenneth W., Jr.1; DuPre, Sally A.1; Sharp, Tiffanny2; Sandmeier, Franziska C.2; Tracy, C. Richard2
通讯作者Hunter, Kenneth W., Jr.
来源期刊JOURNAL OF MICROBIOLOGICAL METHODS
ISSN0167-7012
EISSN1872-8359
出版年2008
卷号75期号:3页码:464-471
英文摘要

Mycoplasma agassizi has been identified as a cause of upper respiratory tract disease (URTD) in the threatened Mojave population of the desert tortoise (Gopherus agassizii), and anti-M. agassizii antibodies have been found by ELISA in as many as 15% of these animals across their geographic range. Here we report that a cohort of 16 egg-reared desert tortoises never exposed to M. agassizii had ELISA antibody titers to this organism that overlapped with titers obtained from some M. agassizii-infected tortoises. These natural antibodies were predominantly of the IgM class. Western blots of plasma from these non-infected tortoises produced a characteristic banding pattern against M. agassizii antigens. A group of 38 wild-caught desert tortoises was tested by ELISA, and although some of these tortoises had antibody titers significantly higher than the non-infected tortoises, there was considerable overlap at the lower titer levels. However, Western blot analysis revealed distinct banding patterns that could readily distinguish between the non-infected tortoises and tortoises with acquired antibodies, regardless of ELISA antibody titers. We conclude that desert tortoises have natural antibodies to M. agassizii that can compromise the determination of infection status by ELISA. However, the Western blot technique can distinguish between natural and acquired antibody patterns and can be used to confirm the diagnosis of M. agassizii infections in the desert tortoise. (C) 2008 Elsevier B.V. All rights reserved.


英文关键词Desert tortoise Natural antibodies IgM Mycoplasma agassizii
类型Article
语种英语
国家USA
收录类别SCI-E
WOS记录号WOS:000261527900014
WOS关键词UPPER RESPIRATORY-TRACT ; LINKED-IMMUNOSORBENT-ASSAY ; IMMUNOGLOBULIN-M ; IGM ANTIBODIES ; DISEASE ; AUTOANTIBODIES ; INFECTION ; SERUM ; PNEUMONIAE ; REPERTOIRE
WOS类目Biochemical Research Methods ; Microbiology
WOS研究方向Biochemistry & Molecular Biology ; Microbiology
资源类型期刊论文
条目标识符http://119.78.100.177/qdio/handle/2XILL650/158326
作者单位1.Univ Nevada, Sch Med, Dept Microbiol & Immunol, Reno, NV 89557 USA;
2.Univ Nevada, Dept Biol, Reno, NV 89557 USA
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GB/T 7714
Hunter, Kenneth W., Jr.,DuPre, Sally A.,Sharp, Tiffanny,et al. Western blot can distinguish natural and acquired antibodies to Mycoplasma agassizii in the desert tortoise (Gopherus agassizii)[J],2008,75(3):464-471.
APA Hunter, Kenneth W., Jr.,DuPre, Sally A.,Sharp, Tiffanny,Sandmeier, Franziska C.,&Tracy, C. Richard.(2008).Western blot can distinguish natural and acquired antibodies to Mycoplasma agassizii in the desert tortoise (Gopherus agassizii).JOURNAL OF MICROBIOLOGICAL METHODS,75(3),464-471.
MLA Hunter, Kenneth W., Jr.,et al."Western blot can distinguish natural and acquired antibodies to Mycoplasma agassizii in the desert tortoise (Gopherus agassizii)".JOURNAL OF MICROBIOLOGICAL METHODS 75.3(2008):464-471.
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