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DOI | 10.1016/j.mimet.2008.07.022 |
Western blot can distinguish natural and acquired antibodies to Mycoplasma agassizii in the desert tortoise (Gopherus agassizii) | |
Hunter, Kenneth W., Jr.1; DuPre, Sally A.1; Sharp, Tiffanny2; Sandmeier, Franziska C.2; Tracy, C. Richard2 | |
通讯作者 | Hunter, Kenneth W., Jr. |
来源期刊 | JOURNAL OF MICROBIOLOGICAL METHODS
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ISSN | 0167-7012 |
EISSN | 1872-8359 |
出版年 | 2008 |
卷号 | 75期号:3页码:464-471 |
英文摘要 | Mycoplasma agassizi has been identified as a cause of upper respiratory tract disease (URTD) in the threatened Mojave population of the desert tortoise (Gopherus agassizii), and anti-M. agassizii antibodies have been found by ELISA in as many as 15% of these animals across their geographic range. Here we report that a cohort of 16 egg-reared desert tortoises never exposed to M. agassizii had ELISA antibody titers to this organism that overlapped with titers obtained from some M. agassizii-infected tortoises. These natural antibodies were predominantly of the IgM class. Western blots of plasma from these non-infected tortoises produced a characteristic banding pattern against M. agassizii antigens. A group of 38 wild-caught desert tortoises was tested by ELISA, and although some of these tortoises had antibody titers significantly higher than the non-infected tortoises, there was considerable overlap at the lower titer levels. However, Western blot analysis revealed distinct banding patterns that could readily distinguish between the non-infected tortoises and tortoises with acquired antibodies, regardless of ELISA antibody titers. We conclude that desert tortoises have natural antibodies to M. agassizii that can compromise the determination of infection status by ELISA. However, the Western blot technique can distinguish between natural and acquired antibody patterns and can be used to confirm the diagnosis of M. agassizii infections in the desert tortoise. (C) 2008 Elsevier B.V. All rights reserved. |
英文关键词 | Desert tortoise Natural antibodies IgM Mycoplasma agassizii |
类型 | Article |
语种 | 英语 |
国家 | USA |
收录类别 | SCI-E |
WOS记录号 | WOS:000261527900014 |
WOS关键词 | UPPER RESPIRATORY-TRACT ; LINKED-IMMUNOSORBENT-ASSAY ; IMMUNOGLOBULIN-M ; IGM ANTIBODIES ; DISEASE ; AUTOANTIBODIES ; INFECTION ; SERUM ; PNEUMONIAE ; REPERTOIRE |
WOS类目 | Biochemical Research Methods ; Microbiology |
WOS研究方向 | Biochemistry & Molecular Biology ; Microbiology |
资源类型 | 期刊论文 |
条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/158326 |
作者单位 | 1.Univ Nevada, Sch Med, Dept Microbiol & Immunol, Reno, NV 89557 USA; 2.Univ Nevada, Dept Biol, Reno, NV 89557 USA |
推荐引用方式 GB/T 7714 | Hunter, Kenneth W., Jr.,DuPre, Sally A.,Sharp, Tiffanny,et al. Western blot can distinguish natural and acquired antibodies to Mycoplasma agassizii in the desert tortoise (Gopherus agassizii)[J],2008,75(3):464-471. |
APA | Hunter, Kenneth W., Jr.,DuPre, Sally A.,Sharp, Tiffanny,Sandmeier, Franziska C.,&Tracy, C. Richard.(2008).Western blot can distinguish natural and acquired antibodies to Mycoplasma agassizii in the desert tortoise (Gopherus agassizii).JOURNAL OF MICROBIOLOGICAL METHODS,75(3),464-471. |
MLA | Hunter, Kenneth W., Jr.,et al."Western blot can distinguish natural and acquired antibodies to Mycoplasma agassizii in the desert tortoise (Gopherus agassizii)".JOURNAL OF MICROBIOLOGICAL METHODS 75.3(2008):464-471. |
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