Knowledge Resource Center for Ecological Environment in Arid Area
DOI | 10.1016/j.jchromb.2008.09.035 |
Simultaneous determination and confirmation of chloramphenicol, thiamphenicol, florfenicol and florfenicol amine in chicken muscle by liquid chromatography-tandem mass spectrometry | |
Zhang, Suxia; Liu, Zhongwei; Guo, Xia; Cheng, Linli; Wang, Zhanhui; Shen, Jianzhong | |
通讯作者 | Shen, Jianzhong |
来源期刊 | JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES |
ISSN | 1570-0232 |
EISSN | 1873-376X |
出版年 | 2008 |
卷号 | 875期号:2页码:399-404 |
英文摘要 | A reliable and sensitive liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) confirmation method has been developed for the simultaneous determination of chloramphenicol (CAP), thiamphenicol (TAP), florfenicol (FF), and florfenicol amine (FFA) in chicken muscle. Samples were extracted with basic ethyl acetate, defatted with hexane. and cleaned tip on Oasis MCX cartridges. LC separation was achieved oil a XTerra C-18 column with gradient elution using a mobile phase composed of acetonitrile and water at a flow rate of 0.20 mL/min. The analysis was carried out on a triple-quadrupole tandem mass spectrometer in the Multiple reaction monitoring (MRM) mode via electrospray interface operated in the positive and negative ionization modes, with deuterated chloramphenicol-d5 (d(5)-CAP) as the internal standard. The method validation was performed according to the criteria of Commission Decision 2002/657/EC. Four identification points were obtained for each analyte with one precursor ion and two product ions. Limits of detection (LODs) were 0.1 mu g/kg for CAP, 0.2 mu g/kg for FF and 1 mu g/kg for TAP and FFA in chicken muscle. Linear calibration curves were obtained over concentration ranges of 0.3-20 mu g/kg for CAP, 0.5-20 mu g/kg for FF and 3-100 mu g/kg for TAP and FFA ill tissues. Mean recoveries of the 4 analytes ranged from 95.1% to 107.3%, with the corresponding intra- and inter-day variation (relative standard deviation, R.S.D.) less than 10.9% and 10.6%, respectively. The decision limit (CC alpha) and detection capability (CC beta) of the method were also reported. (c) 2008 Elsevier B.V. All rights reserved. |
英文关键词 | Chloramphenicol Thiamphenicol Florfenicol Florfenicol amine Liquid chromatography-tandem mass spectrometry Chicken muscle |
类型 | Article |
语种 | 英语 |
国家 | Peoples R China |
收录类别 | SCI-E |
WOS记录号 | WOS:000261272900010 |
WOS关键词 | GAS-CHROMATOGRAPHY ; RESIDUES ; FISH ; VALIDATION ; RESISTANCE ; SHRIMP ; ASSAY ; YOLK |
WOS类目 | Biochemical Research Methods ; Chemistry, Analytical |
WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
来源机构 | 中国农业大学 |
资源类型 | 期刊论文 |
条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/158071 |
作者单位 | China Agr Univ, Coll Vet Med, Dept Vet Pharmacol & Toxicol, Beijing 100094, Peoples R China |
推荐引用方式 GB/T 7714 | Zhang, Suxia,Liu, Zhongwei,Guo, Xia,et al. Simultaneous determination and confirmation of chloramphenicol, thiamphenicol, florfenicol and florfenicol amine in chicken muscle by liquid chromatography-tandem mass spectrometry[J]. 中国农业大学,2008,875(2):399-404. |
APA | Zhang, Suxia,Liu, Zhongwei,Guo, Xia,Cheng, Linli,Wang, Zhanhui,&Shen, Jianzhong.(2008).Simultaneous determination and confirmation of chloramphenicol, thiamphenicol, florfenicol and florfenicol amine in chicken muscle by liquid chromatography-tandem mass spectrometry.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,875(2),399-404. |
MLA | Zhang, Suxia,et al."Simultaneous determination and confirmation of chloramphenicol, thiamphenicol, florfenicol and florfenicol amine in chicken muscle by liquid chromatography-tandem mass spectrometry".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 875.2(2008):399-404. |
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