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We describe a simple and sensitive method for the determination of iodie (I-) in iodine-enriched eggs (IEs) by high-performance liquid chromatography (HPLC). I- in a 0.05 g portion of egg yolk and 0.5 g of egg white of IEs were extracted by a solid-phase extraction (SPE) procedure with a cation-exchange and anion-exchange SPE cartridges (Oasis MCX and Oasis WAX). The I- extracts were evaporated to dryness under a vacuum, and the residues were reconstituted in an HPLC mobile phase to prepare 1 mL of analytical sample solutions. A 10 mu L aliquot of each sample solution was analyzed by ion-pair reversed-phase HPLC with a UV detector at 225 nm. The method gives good sample clean-up. The relative standard deviation for the determination of I-, n = 4, in IE yolk and IE white were 0.8 and 5.5%, respectively. The recoveries of 50 mu g of I- spiked per g of IE yolk and 2.5 mu g of I- spiked per g of IE white were 92.4 and 96.8%, respectively. The detection limits for I-, based on S/N= 3, were 0.76 mu g per g of IE yolk and 0.08 mu g per g of IE white. We applied successfully the method for the determination of I- in commercial IEs.