干旱区生态环境知识资源中心(Arid): Genetic diversity analysis of elite pearl millet inbred lines using RAPID and SSR markers

Arid

DOI	10.1007/BF03321924
	Genetic diversity analysis of elite pearl millet inbred lines using RAPID and SSR markers
	Chandra-Shekara, A. C.; Prasanna, M.; Bhat, S. R.; Singh, B. B.
通讯作者	Prasanna, M.
来源期刊	JOURNAL OF PLANT BIOCHEMISTRY AND BIOTECHNOLOGY
ISSN	0971-7811
出版年	2007
卷号	16 期号:1 页码:23-28
英文摘要	Pearl millet [Pennisetum glaucurn (L) R Br] is one of the widely grown cereal crops in the arid and semi-arid regions of Africa and India. We undertook a study to ascertain the genetic diversity in 21 elite inbreds (parental lines of 13 pearl millet hybrids in India) using 20 Random Amplified Polymorphic DNA (RAPD) and 21 Simple Sequence Repeat (SSR) markers. Based on Polymorphism Information Content (PIC) and unique banding profiles, 6 RAPD primers OPD12, OPA16, OPB6, OPA19, OPB5 and OPB1, and 3 SSR markers Xpsmp2208, Xpsmp2223 and Xpsmp2220, were found to be highly discriminative. The PIC values ranged from 0.28 to 0.48 for the RAPD and from 0.24 to 0.60 for the SSR markers. Cluster analysis and principal component analysis of the combined dataset of RAPD and SSR markers indicated moderate genetic divergence among the elite pearl millet germplasm, besides unraveling the genetic relationships among the male sterile lines and the restorers.
英文关键词	DNA fingerprinting genetic diversity Pennisetum glaucum RAPD SSR
类型	Article
语种	英语
国家	India
收录类别	SCI-E
WOS记录号	WOS:000244581500004
WOS关键词	SEQUENCE REPEAT MARKERS ; PENNISETUM-GLAUCUM ; MICROSATELLITE MARKERS ; L. ; POLYMORPHISMS ; IDENTIFICATION ; GERMPLASM ; PRIMERS ; PLANTS ; AFLP
WOS类目	Biochemistry & Molecular Biology ; Plant Sciences
WOS研究方向	Biochemistry & Molecular Biology ; Plant Sciences
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/155216
作者单位	(1)Indian Agr Res Inst, Div Genet, New Delhi 110012, India;(2)Indian Agr Res Inst, Natl Res Ctr Plant Biotechnol, New Delhi 110012, India
推荐引用方式 GB/T 7714	Chandra-Shekara, A. C.,Prasanna, M.,Bhat, S. R.,et al. Genetic diversity analysis of elite pearl millet inbred lines using RAPID and SSR markers[J],2007,16(1):23-28.
APA	Chandra-Shekara, A. C.,Prasanna, M.,Bhat, S. R.,&Singh, B. B..(2007).Genetic diversity analysis of elite pearl millet inbred lines using RAPID and SSR markers.JOURNAL OF PLANT BIOCHEMISTRY AND BIOTECHNOLOGY,16(1),23-28.
MLA	Chandra-Shekara, A. C.,et al."Genetic diversity analysis of elite pearl millet inbred lines using RAPID and SSR markers".JOURNAL OF PLANT BIOCHEMISTRY AND BIOTECHNOLOGY 16.1(2007):23-28.