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Glycoproteins play important roles in insect physiology. Infection with pathogen always results in the differential expression of some glycoproteins, which may be involved in host-pathogen interactions. In this report, differentially-expressed glycoproteins from the hemolymph of locusts infected with Matarhizium anisopliae were analyzed by two-dimensional electrophoresis (2-DE) and PDQuest software. The results showed that 13 spots were differentially expressed of which nine spots were upregulated and four were downregulated. Using MS/MS with de novo sequencing and NCB1 database searches, three upregulated proteins were identified as locust transferrin, apolipo-protein precursor, and hexarneric storage protein 3. These proteins have been reported to be involved in the insect innate immune response to microbial challenge. Due to the limited available Genome information and protein sequences of locusts, the possible functions of the other 10 differentialy-expressed spots remain unknown. (C) 2007 Elsevier Inc. All riLyhts reserved.