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The aim of this pilot study was to estimate the seroprevalence to Borrelia burgdorferi in dogs from a Mexico-U.S. border region. A total of 94 dogs were tested by Borrelia burgdorferi ELISA kit. Borreliosis or Lyme desease is a worldwide zoonotic tick-borne disease caused by the espirochete Borrelia burgdorferi This disease is characterized by arthritis, lameness, erythema migrans, fatigue, anorexia, general malaise, muscle pain, stiff neck, fever, heart block, kidney failure and neurological changes such as seizures, aggression. In some cases it is cause of death. The resullts show an adjusted prevalence to Borrelia burgdorferi of 8.2% (95% I.C. 1.5-13.3%), obtained using Rogan-Gladen estimator. Since B. burgdorferi is transmitted by ticks, preventive and control measures to erradicate ticks have to be established in order of minimize the risk of infection.