Arid
DOI10.1002/arch.20176
New isoforms of odorant-binding proteins and potential semiochemicals of locusts
Yu, Yanxue; Cui, Xiaojie; Jiang, Quanyong; Jin, Xin; Guo, Zhiyong; Zhao, Xingbo; Bi, Yuping; Zhang, Long
通讯作者Zhang, Long
来源期刊ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY
ISSN0739-4462
EISSN1520-6327
出版年2007
卷号65期号:1页码:39-49
英文摘要

To obtain more information on the elements of chemical communication in the migratory locust (Locusto migratoria) (Orthoptera: Acrididae), we have searched for additional odorant-binding proteins (OBPs) and for volatiles in the feces that could represent potential semiochemicals for this species. A two-dimensional electrophoretic (2DE) analysis of an antennal extract showed only three closely positioned spots that were recognized by the antiserum against locust OBP. Three genes were also identified using PCR and 5’RACE-PCR approaches, encoding isoforms differing from each other for a single amino acid substitution. The gas-chromatographic-electroantennogram (GC-EAD) headspace analysis of a feces sample revealed the presence of several compounds that elicited dose-dependent electrophysiological responses in the antennae of both sexes. Most of these compounds are different from those identified in the feces of the desert locust (Schistocerco gregaria) and reported to be behaviorally active. Ligand-binding experiments performed with such volatiles and recombinant OBP did not show affinity, thus indicating that the binding pocket of OBP requires larger molecules than those so for identified.


英文关键词odorant-binding proteins two-dimensional electrophoresis Locusta migratoria manilensis fecal volatiles EAG
类型Article
语种英语
国家Peoples R China
收录类别SCI-E
WOS记录号WOS:000246050000005
WOS关键词GREGARIA FORSKAL ORTHOPTERA ; SILKMOTH BOMBYX-MORI ; SCHISTOCERCA-GREGARIA ; DESERT LOCUST ; CHEMOSENSORY PROTEINS ; CHEMICAL COMMUNICATION ; PHEROMONE RECEPTION ; DROSOPHILA-ANTENNA ; SOLUBLE-PROTEINS ; EXPRESSION
WOS类目Biochemistry & Molecular Biology ; Entomology ; Physiology
WOS研究方向Biochemistry & Molecular Biology ; Entomology ; Physiology
来源机构中国农业大学
资源类型期刊论文
条目标识符http://119.78.100.177/qdio/handle/2XILL650/153510
作者单位(1)China Agr Univ, Minist Agr, Key Lab Biocontrol Pests, Beijing 100094, Peoples R China;(2)China Agr Univ, Natl Key Lab Agr Biotechnol, Beijing 100094, Peoples R China;(3)Acad Agr, Ctr Biotechnol, Shandong, Peoples R China
推荐引用方式
GB/T 7714
Yu, Yanxue,Cui, Xiaojie,Jiang, Quanyong,et al. New isoforms of odorant-binding proteins and potential semiochemicals of locusts[J]. 中国农业大学,2007,65(1):39-49.
APA Yu, Yanxue.,Cui, Xiaojie.,Jiang, Quanyong.,Jin, Xin.,Guo, Zhiyong.,...&Zhang, Long.(2007).New isoforms of odorant-binding proteins and potential semiochemicals of locusts.ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY,65(1),39-49.
MLA Yu, Yanxue,et al."New isoforms of odorant-binding proteins and potential semiochemicals of locusts".ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY 65.1(2007):39-49.
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