Knowledge Resource Center for Ecological Environment in Arid Area
DOI | 10.1002/arch.20176 |
New isoforms of odorant-binding proteins and potential semiochemicals of locusts | |
Yu, Yanxue; Cui, Xiaojie; Jiang, Quanyong; Jin, Xin; Guo, Zhiyong; Zhao, Xingbo; Bi, Yuping; Zhang, Long | |
通讯作者 | Zhang, Long |
来源期刊 | ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY
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ISSN | 0739-4462 |
EISSN | 1520-6327 |
出版年 | 2007 |
卷号 | 65期号:1页码:39-49 |
英文摘要 | To obtain more information on the elements of chemical communication in the migratory locust (Locusto migratoria) (Orthoptera: Acrididae), we have searched for additional odorant-binding proteins (OBPs) and for volatiles in the feces that could represent potential semiochemicals for this species. A two-dimensional electrophoretic (2DE) analysis of an antennal extract showed only three closely positioned spots that were recognized by the antiserum against locust OBP. Three genes were also identified using PCR and 5’RACE-PCR approaches, encoding isoforms differing from each other for a single amino acid substitution. The gas-chromatographic-electroantennogram (GC-EAD) headspace analysis of a feces sample revealed the presence of several compounds that elicited dose-dependent electrophysiological responses in the antennae of both sexes. Most of these compounds are different from those identified in the feces of the desert locust (Schistocerco gregaria) and reported to be behaviorally active. Ligand-binding experiments performed with such volatiles and recombinant OBP did not show affinity, thus indicating that the binding pocket of OBP requires larger molecules than those so for identified. |
英文关键词 | odorant-binding proteins two-dimensional electrophoresis Locusta migratoria manilensis fecal volatiles EAG |
类型 | Article |
语种 | 英语 |
国家 | Peoples R China |
收录类别 | SCI-E |
WOS记录号 | WOS:000246050000005 |
WOS关键词 | GREGARIA FORSKAL ORTHOPTERA ; SILKMOTH BOMBYX-MORI ; SCHISTOCERCA-GREGARIA ; DESERT LOCUST ; CHEMOSENSORY PROTEINS ; CHEMICAL COMMUNICATION ; PHEROMONE RECEPTION ; DROSOPHILA-ANTENNA ; SOLUBLE-PROTEINS ; EXPRESSION |
WOS类目 | Biochemistry & Molecular Biology ; Entomology ; Physiology |
WOS研究方向 | Biochemistry & Molecular Biology ; Entomology ; Physiology |
来源机构 | 中国农业大学 |
资源类型 | 期刊论文 |
条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/153510 |
作者单位 | (1)China Agr Univ, Minist Agr, Key Lab Biocontrol Pests, Beijing 100094, Peoples R China;(2)China Agr Univ, Natl Key Lab Agr Biotechnol, Beijing 100094, Peoples R China;(3)Acad Agr, Ctr Biotechnol, Shandong, Peoples R China |
推荐引用方式 GB/T 7714 | Yu, Yanxue,Cui, Xiaojie,Jiang, Quanyong,et al. New isoforms of odorant-binding proteins and potential semiochemicals of locusts[J]. 中国农业大学,2007,65(1):39-49. |
APA | Yu, Yanxue.,Cui, Xiaojie.,Jiang, Quanyong.,Jin, Xin.,Guo, Zhiyong.,...&Zhang, Long.(2007).New isoforms of odorant-binding proteins and potential semiochemicals of locusts.ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY,65(1),39-49. |
MLA | Yu, Yanxue,et al."New isoforms of odorant-binding proteins and potential semiochemicals of locusts".ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY 65.1(2007):39-49. |
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