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Herein is reported, for the first time, a simple and reliable chiral reversed-phase liquid chromatographic method coupled to ultraviolet (UV) detection for simultaneous determination of eslicarbazepine acetate (ESL) and its metabolites, S-licarbazepine (S-LC), R-licarbazepine (R-LC) and oxcarbazepine (OXC), in mouse plasma and brain, liver and kidney tissue homogenates. All analytes and the internal standard were extracted from plasma and tissue homogenates by a solid-phase extraction procedure using Waters Oasis hydrophilic-lipophilic balance cartridges. The chromatographic separation was performed by isocratic elution with water/methanol (88:12, v/v), pumped at a flow rate of 0.7 mL min(-1), on a Lichro-CART 250-4 ChiraDex (beta-cyclodextrin, 5 mu m) column at 30 degrees C. The UV detector was set at 225 nm. Calibration curves were linear (r(2) >= 0.996) in the ranges 0.4-8 mu g mL(-1), 0.1-1.5 mu g mL(-1) and 0.1-2 mu g mL(-1) for ESL and OXC and in the ranges 0.4-80 mu g mL(-1), 0.1-15 mu g mL(-1) and 0.1-20 mu g mL(-1) for R-LC and S-LC in plasma, brain and liver/kidney homogenates, respectively. The overall precision not exceeded 11.6% (%CV) and the accuracy ranged from -3.79 to 3.84% (%bias), considering all analytes in all matrices. Hence, this method will be a useful tool to characterize the pharmacokinetic disposition of ESL in mice. (c) 2007 Elsevier B.V. All rights reserved.