Knowledge Resource Center for Ecological Environment in Arid Area
DOI | 10.1016/j.toxicon.2006.07.030 |
Assessment of fitness for purpose of an insect bioassay using the desert locust (Schistocerca gregaria L.) for the detection of paralytic shellfish toxins in shellfish flesh | |
Cook, A. C.; Morris, S.; Reese, R. A.; Irving, S. N. | |
通讯作者 | Cook, A. C. |
来源期刊 | TOXICON |
ISSN | 0041-0101 |
出版年 | 2006 |
卷号 | 48期号:6页码:662-671 |
英文摘要 | We have developed a bioassay using 5th instar desert locusts (Schistocerca gregaria L.) for the detection of saxitoxin-the paralytic shellfish poison in shellfish flesh. The bioassay procedure is to inject 10 locusts with a shellfish extract, and assess their conditions at time points up to 2 h post injection, looking for an endpoint of paralysis. From the proportion responding, the equivalent dose of pure saxitoxin could be estimated. Performance characteristics of the bioassay were assessed using shellfish samples spiked with saxitoxin, and we found the bioassay could detect and quantify toxin levels in the range of regulatory relevance. Relative toxicities of selected saxitoxin analogues differed from those reported in mammalian systems. Variation for repeatability conditions was acceptable but variation was higher under reproducibility conditions. This was related to (a) batches of insects from different suppliers, (b) different operators, and (c) different observers assessing the endpoint. We also noted adverse reactions with some shellfish species. These problems may be resolved by further refinement of the method and operator training, before formal validation. Nevertheless, we suggest the method potentially offers a simple, ethically acceptable, broad-specificity functional bioassay, which is desirable in any toxin-monitoring programme. Crown Copyright (c) 2006 Published by Elsevier Ltd. All rights reserved. |
英文关键词 | paralytic shellfish poisoning PSP saxitoxin bioassay desert locust Schistocerca gregaria |
类型 | Article |
语种 | 英语 |
国家 | England |
收录类别 | SCI-E |
WOS记录号 | WOS:000241937200007 |
WOS关键词 | POISONING TOXINS ; PRECHROMATOGRAPHIC OXIDATION ; QUANTITATIVE-DETERMINATION ; FLUORESCENCE DETECTION ; LIQUID-CHROMATOGRAPHY ; MOUSE BIOASSAY ; PSP TOXINS ; TISSUE |
WOS类目 | Pharmacology & Pharmacy ; Toxicology |
WOS研究方向 | Pharmacology & Pharmacy ; Toxicology |
资源类型 | 期刊论文 |
条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/153132 |
作者单位 | (1)Weymouth Lab, Ctr Environm Fisheries & Aquaculture Sci, Weymouth DT4 8UB, Dorset, England;(2)Ctr Environm Fisheries & Aquaculture Sci, Burnham On Crouch, Essex, England |
推荐引用方式 GB/T 7714 | Cook, A. C.,Morris, S.,Reese, R. A.,et al. Assessment of fitness for purpose of an insect bioassay using the desert locust (Schistocerca gregaria L.) for the detection of paralytic shellfish toxins in shellfish flesh[J],2006,48(6):662-671. |
APA | Cook, A. C.,Morris, S.,Reese, R. A.,&Irving, S. N..(2006).Assessment of fitness for purpose of an insect bioassay using the desert locust (Schistocerca gregaria L.) for the detection of paralytic shellfish toxins in shellfish flesh.TOXICON,48(6),662-671. |
MLA | Cook, A. C.,et al."Assessment of fitness for purpose of an insect bioassay using the desert locust (Schistocerca gregaria L.) for the detection of paralytic shellfish toxins in shellfish flesh".TOXICON 48.6(2006):662-671. |
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