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Under semi-arid conditions, the properties of many soils are influenced by the presence of organic matter and calcium carbonate (CaCO3). However, the influence of different tillage systems on the development of these properties has scarcely been studied under semi-arid Mediterranean conditions. We studied the effect of long-term conservation tillage (CT) and traditional tillage (TT) on the stratification ratio of soil organic carbon and on CaCO3 content. The study was conducted in a wheat (Triticum aestivum L.)-sunflower (Helianthus annuus L.) crop rotation established in 1991 under tainted conditions in Southwestern Spain. As is traditional in this area, wheat was fertilised, but sunflower was not. Conservation tillage was characterised by reduced number of tillage operations and leaving crop residues on the soil surface, while TT was with mouldboard ploughing. Stratification ratio of soil organic C was calculated from C contents in the 0-5 and 5-10 cm soil layers divided by that in the 2540 cm. Stratification ratio of soil organic C under the CT (>2) was significantly greater than under TT (<2); values >2 indicating better soil quality. Our results show a loss of CaCO3 under both tillage systems. However, the loss of CaCO3 was significantly higher under TT than under CT. Also, P and K accumulated in the soil surface and stratification ratio for both nutrients was greater in CT than in TT. (C) 2005 Elsevier B.V. All rights reserved.