Arid
DOI10.1007/s11120-005-9010-3
Simultaneous collection of rapid chlorophyll fluorescence induction kinetics, fluorescence quenching parameters, and environmental data using an automated PAM-2000/CR10X data logging system
Gray, DW; Cardon, ZG; Lewis, LA
通讯作者Gray, DW
来源期刊PHOTOSYNTHESIS RESEARCH
ISSN0166-8595
出版年2006
卷号87期号:3页码:295-301
英文摘要

The PAM-2000 portable chlorophyll fluorometer represents one of the first commercially available instruments utilizing the Pulse Amplitude Modulation (PAM) measurement principle, and has become a widely used platform for measuring chlorophyll fluorescence in a wide range of study systems. In this paper, we describe a new method for externally driving and gathering data from the PAM-2000, a method that allows the user to execute a pre-defined user run (or runs) and capture (1) rapid induction kinetics (at 2 ms frequency) during all saturating pulses, (2) measures of F, F-o, F-o’, F-m, and F-m’ associated with those same pulses, and (3) changes in fluorescence F at user-defined intervals between pulses, for the entire user run, with all data compressed into a single, manageable data logger file. Practically, the method makes possible, for example, a post-hoc evaluation of the appropriateness of saturation pulse lengths and intensities during a user run. More importantly it captures, during entire user runs, the varied information contained in slow changes in fluorescence between saturating pulses, as well as rapid induction kinetics, quenching coefficients and quantum yields all gathered simultaneously from all saturating flashes.


英文关键词automated data collection chlorophyll fluorescence desert algae fluorescence induction kinetics PAM-2000 P-peak shift desiccation/rehydration
类型Article
语种英语
国家USA
收录类别SCI-E
WOS记录号WOS:000237510700007
WOS关键词ALGAE
WOS类目Plant Sciences
WOS研究方向Plant Sciences
资源类型期刊论文
条目标识符http://119.78.100.177/qdio/handle/2XILL650/152721
作者单位(1)Univ Connecticut, Dept Ecol & Evolutionary Biol, Storrs, CT 06269 USA
推荐引用方式
GB/T 7714
Gray, DW,Cardon, ZG,Lewis, LA. Simultaneous collection of rapid chlorophyll fluorescence induction kinetics, fluorescence quenching parameters, and environmental data using an automated PAM-2000/CR10X data logging system[J],2006,87(3):295-301.
APA Gray, DW,Cardon, ZG,&Lewis, LA.(2006).Simultaneous collection of rapid chlorophyll fluorescence induction kinetics, fluorescence quenching parameters, and environmental data using an automated PAM-2000/CR10X data logging system.PHOTOSYNTHESIS RESEARCH,87(3),295-301.
MLA Gray, DW,et al."Simultaneous collection of rapid chlorophyll fluorescence induction kinetics, fluorescence quenching parameters, and environmental data using an automated PAM-2000/CR10X data logging system".PHOTOSYNTHESIS RESEARCH 87.3(2006):295-301.
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