Knowledge Resource Center for Ecological Environment in Arid Area
DOI | 10.1007/s11120-005-9010-3 |
Simultaneous collection of rapid chlorophyll fluorescence induction kinetics, fluorescence quenching parameters, and environmental data using an automated PAM-2000/CR10X data logging system | |
Gray, DW; Cardon, ZG; Lewis, LA | |
通讯作者 | Gray, DW |
来源期刊 | PHOTOSYNTHESIS RESEARCH
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ISSN | 0166-8595 |
出版年 | 2006 |
卷号 | 87期号:3页码:295-301 |
英文摘要 | The PAM-2000 portable chlorophyll fluorometer represents one of the first commercially available instruments utilizing the Pulse Amplitude Modulation (PAM) measurement principle, and has become a widely used platform for measuring chlorophyll fluorescence in a wide range of study systems. In this paper, we describe a new method for externally driving and gathering data from the PAM-2000, a method that allows the user to execute a pre-defined user run (or runs) and capture (1) rapid induction kinetics (at 2 ms frequency) during all saturating pulses, (2) measures of F, F-o, F-o’, F-m, and F-m’ associated with those same pulses, and (3) changes in fluorescence F at user-defined intervals between pulses, for the entire user run, with all data compressed into a single, manageable data logger file. Practically, the method makes possible, for example, a post-hoc evaluation of the appropriateness of saturation pulse lengths and intensities during a user run. More importantly it captures, during entire user runs, the varied information contained in slow changes in fluorescence between saturating pulses, as well as rapid induction kinetics, quenching coefficients and quantum yields all gathered simultaneously from all saturating flashes. |
英文关键词 | automated data collection chlorophyll fluorescence desert algae fluorescence induction kinetics PAM-2000 P-peak shift desiccation/rehydration |
类型 | Article |
语种 | 英语 |
国家 | USA |
收录类别 | SCI-E |
WOS记录号 | WOS:000237510700007 |
WOS关键词 | ALGAE |
WOS类目 | Plant Sciences |
WOS研究方向 | Plant Sciences |
资源类型 | 期刊论文 |
条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/152721 |
作者单位 | (1)Univ Connecticut, Dept Ecol & Evolutionary Biol, Storrs, CT 06269 USA |
推荐引用方式 GB/T 7714 | Gray, DW,Cardon, ZG,Lewis, LA. Simultaneous collection of rapid chlorophyll fluorescence induction kinetics, fluorescence quenching parameters, and environmental data using an automated PAM-2000/CR10X data logging system[J],2006,87(3):295-301. |
APA | Gray, DW,Cardon, ZG,&Lewis, LA.(2006).Simultaneous collection of rapid chlorophyll fluorescence induction kinetics, fluorescence quenching parameters, and environmental data using an automated PAM-2000/CR10X data logging system.PHOTOSYNTHESIS RESEARCH,87(3),295-301. |
MLA | Gray, DW,et al."Simultaneous collection of rapid chlorophyll fluorescence induction kinetics, fluorescence quenching parameters, and environmental data using an automated PAM-2000/CR10X data logging system".PHOTOSYNTHESIS RESEARCH 87.3(2006):295-301. |
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