Arid
DOI10.1016/j.jpba.2006.04.031
Simple determination of the HIV protease inhibitor atazanavir in human plasma by high-performance liquid chromatography with UV detection
Loregian, Arianna; Pagni, Silvana; Ballarin, Elisa; Sinigalia, Elisa; Parisi, Saverio Giuseppe; Palu, Giorgio
通讯作者Palu, Giorgio
来源期刊JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
ISSN0731-7085
出版年2006
卷号42期号:4页码:500-505
英文摘要

A simple high-performance liquid chromatography method for the determination of the human immunodeficiency virus protease inhibitor atazanavir in human plasma samples was developed and validated. The method involved a rapid and simple solid-phase extraction of atazanavir using Oasis HLB 1 cc cartridges, an isocratic reversed-phase liquid chromatography on an XTerra RP 18 (150 mm x 4.6 mm, 3.5 mu m) column, and ultraviolet detection at 203 nm. The mobile phase consisted of phosphate buffer (pH 6, 52.5 mM) and acetonitrile (43:57, v/v). Up to 48 samples could be measured in one day since the run-time of one sample was 30 min. The assay was linear from 0.04 to 10 mu g/ml with a lower limit of quantification of 0.04 mu g/ml. The mean absolute recovery of ATV was 98.1%. The method was precise, with both intra-day and inter-day coefficients of variation <= 3.0%, and accurate (deviations ranged from -3.0% to 4.5% and from -3.6% to 4.7% for intra-day and inter-day analysis, respectively). There was no interference from 35 tested potentially co-administrated drugs. This method provides a simple, sensitive, precise and reproducible assay for the therapeutic drug monitoring of atazanavir in clinical routine of laboratories with standard equipment. (c) 2006 Elsevier B.V. All rights reserved.


英文关键词atazanavir BMS-232632 HIV protease inhibitors solid-phase extraction high-performance liquid chromatography therapeutic drug monitoring
类型Article
语种英语
国家Italy
收录类别SCI-E
WOS记录号WOS:000241125900014
WOS关键词TANDEM MASS-SPECTROMETRY ; SOLID-PHASE EXTRACTION ; REVERSE-TRANSCRIPTASE INHIBITORS ; SIMULTANEOUS QUANTITATIVE ASSAY ; ANTIRETROVIRAL DRUGS ; AMPRENAVIR ; SAQUINAVIR ; METABOLITE ; NELFINAVIR ; EFAVIRENZ
WOS类目Chemistry, Analytical ; Pharmacology & Pharmacy
WOS研究方向Chemistry ; Pharmacology & Pharmacy
资源类型期刊论文
条目标识符http://119.78.100.177/qdio/handle/2XILL650/152317
作者单位(1)Univ Padua, Dept Hist Microbiol & Med Biotechnol, I-35121 Padua, Italy;(2)Padua Univ Hosp, Clin Microbiol & Virol Unit, I-35121 Padua, Italy
推荐引用方式
GB/T 7714
Loregian, Arianna,Pagni, Silvana,Ballarin, Elisa,et al. Simple determination of the HIV protease inhibitor atazanavir in human plasma by high-performance liquid chromatography with UV detection[J],2006,42(4):500-505.
APA Loregian, Arianna,Pagni, Silvana,Ballarin, Elisa,Sinigalia, Elisa,Parisi, Saverio Giuseppe,&Palu, Giorgio.(2006).Simple determination of the HIV protease inhibitor atazanavir in human plasma by high-performance liquid chromatography with UV detection.JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,42(4),500-505.
MLA Loregian, Arianna,et al."Simple determination of the HIV protease inhibitor atazanavir in human plasma by high-performance liquid chromatography with UV detection".JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 42.4(2006):500-505.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Loregian, Arianna]的文章
[Pagni, Silvana]的文章
[Ballarin, Elisa]的文章
百度学术
百度学术中相似的文章
[Loregian, Arianna]的文章
[Pagni, Silvana]的文章
[Ballarin, Elisa]的文章
必应学术
必应学术中相似的文章
[Loregian, Arianna]的文章
[Pagni, Silvana]的文章
[Ballarin, Elisa]的文章
相关权益政策
暂无数据
收藏/分享

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。