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DOI | 10.1016/j.jpba.2006.04.031 |
Simple determination of the HIV protease inhibitor atazanavir in human plasma by high-performance liquid chromatography with UV detection | |
Loregian, Arianna; Pagni, Silvana; Ballarin, Elisa; Sinigalia, Elisa; Parisi, Saverio Giuseppe; Palu, Giorgio | |
通讯作者 | Palu, Giorgio |
来源期刊 | JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
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ISSN | 0731-7085 |
出版年 | 2006 |
卷号 | 42期号:4页码:500-505 |
英文摘要 | A simple high-performance liquid chromatography method for the determination of the human immunodeficiency virus protease inhibitor atazanavir in human plasma samples was developed and validated. The method involved a rapid and simple solid-phase extraction of atazanavir using Oasis HLB 1 cc cartridges, an isocratic reversed-phase liquid chromatography on an XTerra RP 18 (150 mm x 4.6 mm, 3.5 mu m) column, and ultraviolet detection at 203 nm. The mobile phase consisted of phosphate buffer (pH 6, 52.5 mM) and acetonitrile (43:57, v/v). Up to 48 samples could be measured in one day since the run-time of one sample was 30 min. The assay was linear from 0.04 to 10 mu g/ml with a lower limit of quantification of 0.04 mu g/ml. The mean absolute recovery of ATV was 98.1%. The method was precise, with both intra-day and inter-day coefficients of variation <= 3.0%, and accurate (deviations ranged from -3.0% to 4.5% and from -3.6% to 4.7% for intra-day and inter-day analysis, respectively). There was no interference from 35 tested potentially co-administrated drugs. This method provides a simple, sensitive, precise and reproducible assay for the therapeutic drug monitoring of atazanavir in clinical routine of laboratories with standard equipment. (c) 2006 Elsevier B.V. All rights reserved. |
英文关键词 | atazanavir BMS-232632 HIV protease inhibitors solid-phase extraction high-performance liquid chromatography therapeutic drug monitoring |
类型 | Article |
语种 | 英语 |
国家 | Italy |
收录类别 | SCI-E |
WOS记录号 | WOS:000241125900014 |
WOS关键词 | TANDEM MASS-SPECTROMETRY ; SOLID-PHASE EXTRACTION ; REVERSE-TRANSCRIPTASE INHIBITORS ; SIMULTANEOUS QUANTITATIVE ASSAY ; ANTIRETROVIRAL DRUGS ; AMPRENAVIR ; SAQUINAVIR ; METABOLITE ; NELFINAVIR ; EFAVIRENZ |
WOS类目 | Chemistry, Analytical ; Pharmacology & Pharmacy |
WOS研究方向 | Chemistry ; Pharmacology & Pharmacy |
资源类型 | 期刊论文 |
条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/152317 |
作者单位 | (1)Univ Padua, Dept Hist Microbiol & Med Biotechnol, I-35121 Padua, Italy;(2)Padua Univ Hosp, Clin Microbiol & Virol Unit, I-35121 Padua, Italy |
推荐引用方式 GB/T 7714 | Loregian, Arianna,Pagni, Silvana,Ballarin, Elisa,et al. Simple determination of the HIV protease inhibitor atazanavir in human plasma by high-performance liquid chromatography with UV detection[J],2006,42(4):500-505. |
APA | Loregian, Arianna,Pagni, Silvana,Ballarin, Elisa,Sinigalia, Elisa,Parisi, Saverio Giuseppe,&Palu, Giorgio.(2006).Simple determination of the HIV protease inhibitor atazanavir in human plasma by high-performance liquid chromatography with UV detection.JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,42(4),500-505. |
MLA | Loregian, Arianna,et al."Simple determination of the HIV protease inhibitor atazanavir in human plasma by high-performance liquid chromatography with UV detection".JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 42.4(2006):500-505. |
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