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DOI10.1016/j.chroma.2006.08.027
Analysis of cytokinin nucleotides in coconut (Cocos nucifera L.) water using capillary zone electrophoresis-tandem mass spectrometry after solid-phase extraction
Ge, Liya; Yong, Jean Wan Hong; Tan, Swee Ngin; Yang, Xin Hao; Ong, Eng Shi
通讯作者Tan, Swee Ngin
来源期刊JOURNAL OF CHROMATOGRAPHY A
ISSN0021-9673
出版年2006
卷号1133期号:1-2页码:322-331
英文摘要

A method based on solid-phase extraction (SPE) and capillary zone electrophoresis-tandem mass spectrometry (CZE-MS/MS) is described for the separation and determination of six cytokinin nucleotides in coconut water. The best CZE separation for the six cytokinin nucleotide standards was achieved using a 25 mM ammonium formate/formic acid buffer (pH 3.8) and 2% (v/v) methanol with an applied gradient separation voltage (25 kV for 32 min, and then a linear gradient to 30 kV in 5 min, finally 30 kV to the end of separation) in less than 60 min. MS/MS with multiple reaction monitoring (MRM) detection was carried out to obtain sufficient selectivity and sensitivity for the cytokinin nucleotides. The combined use of on-line sample stacking and CZE-MS/MS achieved limits of detection (LODs) in the range of 0.06-0.19 mu M for the six cytokinin nucleotides at a signal-to-noise ratio of 3. Furthermore, a novel dual-step SPE procedure was developed for the pre-concentration and purification of cytokinin nucleotides using Oasis HLB and Oasis MAX cartridges. The recoveries of the cytokinin nucleotides after the dual-step SPE were in the range of 44-71%. The combination of off-line SPE, on-line sample stacking and CZE-MS/MS approach was successfully applied to screen for endogenous cytokinin nucleotides present in coconut water sample. trans-Zeatin riboside-5’-monophosphate (ZMP) was detected and quantified in coconut water by CZE-MS/MS after SPE and on-line sample stacking. (c) 2006 Elsevier B.V. All rights reserved.


英文关键词capillary zone electrophoresis tandem mass spectrometry cytokinin nucleotide solid-phase extraction coconut water
类型Article
语种英语
国家Singapore
收录类别SCI-E
WOS记录号WOS:000241772800037
WOS关键词MICELLAR ELECTROKINETIC CHROMATOGRAPHY ; IN-VITRO ; INHIBITORY-ACTIVITY ; ABSCISIC-ACID ; SEPARATION ; KINETIN ; BIOSYNTHESIS ; OPTIMIZATION ; FIBROBLASTS ; ARABIDOPSIS
WOS类目Biochemical Research Methods ; Chemistry, Analytical
WOS研究方向Biochemistry & Molecular Biology ; Chemistry
资源类型期刊论文
条目标识符http://119.78.100.177/qdio/handle/2XILL650/152062
作者单位(1)Nanyang Technol Univ, Nat Sci & Sci Educ Acad Grp, Singapore 637616, Singapore;(2)Singapore Epson Ind Pte Ltd, Dept Engn, Plating Div, Singapore 628162, Singapore;(3)Natl Univ Singapore, Yong Loo Ling Sch Med, Dept Community Occupat & Family Med, Singapore 117597, Singapore
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GB/T 7714
Ge, Liya,Yong, Jean Wan Hong,Tan, Swee Ngin,et al. Analysis of cytokinin nucleotides in coconut (Cocos nucifera L.) water using capillary zone electrophoresis-tandem mass spectrometry after solid-phase extraction[J],2006,1133(1-2):322-331.
APA Ge, Liya,Yong, Jean Wan Hong,Tan, Swee Ngin,Yang, Xin Hao,&Ong, Eng Shi.(2006).Analysis of cytokinin nucleotides in coconut (Cocos nucifera L.) water using capillary zone electrophoresis-tandem mass spectrometry after solid-phase extraction.JOURNAL OF CHROMATOGRAPHY A,1133(1-2),322-331.
MLA Ge, Liya,et al."Analysis of cytokinin nucleotides in coconut (Cocos nucifera L.) water using capillary zone electrophoresis-tandem mass spectrometry after solid-phase extraction".JOURNAL OF CHROMATOGRAPHY A 1133.1-2(2006):322-331.
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