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DOI | 10.1111/j.1742-4658.2006.05204.x |
Local binding with globally distributed changes in a small protease inhibitor upon enzyme binding | |
Gaspari, Z; Szenthe, B; Patthy, A; Westler, WM; Graf, L; Perczel, A | |
通讯作者 | Perczel, A |
来源期刊 | FEBS JOURNAL
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ISSN | 1742-464X |
出版年 | 2006 |
卷号 | 273期号:8页码:1831-1842 |
英文摘要 | Complexation of the small serine protease inhibitor Schistocerca gregaria chymotrypsin inhibitor (SGCI), a member of the pacifastin inhibitor family, with bovine chymotrypsin was followed by NMR spectroscopy. (1)H-(15)N correlation (HSQC) spectra of the inhibitor with increasing amounts of the enzyme reveal tight and specific binding in agreement with biochemical data. Unexpectedly, and unparalleled among canonical serine protease inhibitors, not only residues in the protease-binding loop of the inhibitor, but also some segments of it located spatially far from the substrate-binding cleft of the enzyme were affected by complexation. However, besides changes, some of the dynamical features of the free inhibitor are retained in the complex. Comparison of the free and complexed inhibitor structures revealed that most, but not all, of the observed chemical shift changes can be attributed to minor structural transitions. We suggest that the classical ’scaffold + binding loop’ model of canonical inhibitors might not be fully valid for the inhibitor family studied. In our view, this feature allows for the emergence of both taxon-specific and nontaxon-specific inhibitors in this group of small proteins. |
英文关键词 | enzyme-inhibitor complex internal dynamics NMR spectroscopy pacifastin inhibitor family SGCI |
类型 | Article |
语种 | 英语 |
国家 | Hungary ; USA |
收录类别 | SCI-E |
WOS记录号 | WOS:000236394100022 |
WOS关键词 | LOCUST SCHISTOCERCA-GREGARIA ; CHEMICAL-SHIFT INDEX ; DESERT LOCUST ; PROTEINASE-INHIBITORS ; CHYMOTRYPSIN INHIBITOR ; SECONDARY STRUCTURE ; NMR-SPECTROSCOPY ; TRYPSIN-INHIBITOR ; BACKBONE DYNAMICS ; ACTIVE-SITE |
WOS类目 | Biochemistry & Molecular Biology |
WOS研究方向 | Biochemistry & Molecular Biology |
资源类型 | 期刊论文 |
条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/151382 |
作者单位 | (1)Eotvos Lorand Univ, Inst Chem, H-1117 Budapest, Hungary;(2)Eotvos Lorand Univ, Inst Biol, Budapest, Hungary;(3)Univ Wisconsin, Natl Magnet Resonance Fac, Madison, WI USA |
推荐引用方式 GB/T 7714 | Gaspari, Z,Szenthe, B,Patthy, A,et al. Local binding with globally distributed changes in a small protease inhibitor upon enzyme binding[J],2006,273(8):1831-1842. |
APA | Gaspari, Z,Szenthe, B,Patthy, A,Westler, WM,Graf, L,&Perczel, A.(2006).Local binding with globally distributed changes in a small protease inhibitor upon enzyme binding.FEBS JOURNAL,273(8),1831-1842. |
MLA | Gaspari, Z,et al."Local binding with globally distributed changes in a small protease inhibitor upon enzyme binding".FEBS JOURNAL 273.8(2006):1831-1842. |
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