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DOI10.1111/j.1742-4658.2006.05204.x
Local binding with globally distributed changes in a small protease inhibitor upon enzyme binding
Gaspari, Z; Szenthe, B; Patthy, A; Westler, WM; Graf, L; Perczel, A
通讯作者Perczel, A
来源期刊FEBS JOURNAL
ISSN1742-464X
出版年2006
卷号273期号:8页码:1831-1842
英文摘要

Complexation of the small serine protease inhibitor Schistocerca gregaria chymotrypsin inhibitor (SGCI), a member of the pacifastin inhibitor family, with bovine chymotrypsin was followed by NMR spectroscopy. (1)H-(15)N correlation (HSQC) spectra of the inhibitor with increasing amounts of the enzyme reveal tight and specific binding in agreement with biochemical data. Unexpectedly, and unparalleled among canonical serine protease inhibitors, not only residues in the protease-binding loop of the inhibitor, but also some segments of it located spatially far from the substrate-binding cleft of the enzyme were affected by complexation. However, besides changes, some of the dynamical features of the free inhibitor are retained in the complex. Comparison of the free and complexed inhibitor structures revealed that most, but not all, of the observed chemical shift changes can be attributed to minor structural transitions. We suggest that the classical ’scaffold + binding loop’ model of canonical inhibitors might not be fully valid for the inhibitor family studied. In our view, this feature allows for the emergence of both taxon-specific and nontaxon-specific inhibitors in this group of small proteins.


英文关键词enzyme-inhibitor complex internal dynamics NMR spectroscopy pacifastin inhibitor family SGCI
类型Article
语种英语
国家Hungary ; USA
收录类别SCI-E
WOS记录号WOS:000236394100022
WOS关键词LOCUST SCHISTOCERCA-GREGARIA ; CHEMICAL-SHIFT INDEX ; DESERT LOCUST ; PROTEINASE-INHIBITORS ; CHYMOTRYPSIN INHIBITOR ; SECONDARY STRUCTURE ; NMR-SPECTROSCOPY ; TRYPSIN-INHIBITOR ; BACKBONE DYNAMICS ; ACTIVE-SITE
WOS类目Biochemistry & Molecular Biology
WOS研究方向Biochemistry & Molecular Biology
资源类型期刊论文
条目标识符http://119.78.100.177/qdio/handle/2XILL650/151382
作者单位(1)Eotvos Lorand Univ, Inst Chem, H-1117 Budapest, Hungary;(2)Eotvos Lorand Univ, Inst Biol, Budapest, Hungary;(3)Univ Wisconsin, Natl Magnet Resonance Fac, Madison, WI USA
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GB/T 7714
Gaspari, Z,Szenthe, B,Patthy, A,et al. Local binding with globally distributed changes in a small protease inhibitor upon enzyme binding[J],2006,273(8):1831-1842.
APA Gaspari, Z,Szenthe, B,Patthy, A,Westler, WM,Graf, L,&Perczel, A.(2006).Local binding with globally distributed changes in a small protease inhibitor upon enzyme binding.FEBS JOURNAL,273(8),1831-1842.
MLA Gaspari, Z,et al."Local binding with globally distributed changes in a small protease inhibitor upon enzyme binding".FEBS JOURNAL 273.8(2006):1831-1842.
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