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A simple, sensitive and selective high-performance liquid chromatography (HPLC) method with ultraviolet detection (305 nm) was developed and validated for quantification of cefditoren (CAS 104145-95-1), a broad-spectrum orally administered ce-phalosporin in human plasma. Following solid-phase extraction using Waters Oasis (TM) SPE cartridges, the analyte and internal standard (hydrochlorothiazide, CAS 58-93-5) were separated using an isocratic mobile phase of 0.03% trifluoro acetic acid buffer I acetonitrile (81/19, v/v) on reverse phase Waters symmetry 0 C-18 column. The lower limit of quantification was 50 ng/mL, with a relative standard deviation of less than 4 %. A linear range of 50 to 5000 ng/mL was established. This HPLC method was validated with between-batch and within-batch precision of 0.5 to 3.7 % and 0.5 to 2.5 %, respectively. The between-batch and within-batch accuracy was 96.9 to 103.8 % and 97.5 to 102.3 %, respectively. Stability of cefditoren in plasma was excellent, with no evidence of degradation during sample processing (autosampler) and 30 days storage in a freezer. This validated method is sensitive, simple and repeatable enough to be used in pharmacokinetic studies.