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DOI | 10.1021/ac061006v |
Multiresidue method for the determination of quinolone antibiotics in bovine raw milk by capillary electrophoresis-tandem mass spectrometry | |
Lara, Francisco J.; Garcia-Campana, Ana M.; Ales-Barrero, Fermin; Bosque-Sendra, Juan M.; Garcia-Ayuso, Luis E. | |
通讯作者 | Garcia-Campana, Ana M. |
来源期刊 | ANALYTICAL CHEMISTRY |
ISSN | 0003-2700 |
EISSN | 1520-6882 |
出版年 | 2006 |
卷号 | 78期号:22页码:7665-7673 |
英文摘要 | A new analytical method based on capillary zone electrophoresis-tandem mass spectrometry (CZE-MS/MS) is proposed and validated for the identification and simultaneous quantification of eight quinolones for veterinary use in bovine raw milk. The studied quinolones include danofloxacin, sarafloxacin, ciprofloxacin, marbofloxacin, enrofloxacin, difloxacin, oxolinic acid, and flumequine, whose contents are regulated by the EU Council Regulation no. 2377/90 in animal edible tissues. Different parameters (i.e., separation buffer composition and electrospray conditions) were optimized in order to obtain both an adequate CE separation and a high sensitivity, using experimental design methodology to consider the interactions among the studied variables. MS/MS experiments using an ion trap as analyzer operating in the multiple reaction monitoring mode were carried out to achieve the minimum number of identification points according to the 2002/657/EC European Decision. For the quantification in bovine raw milk samples, a two-step solid-phase extraction procedure was developed using Oasis MAX and HLB cartridges without protein precipitation. Satisfactory results were obtained in terms of linearity (r(2) between 0.989 and 0.992) and precision (RSD below 18%). The limits of detection and quantification ( below 6 and 24 ppb, respectively) were in all cases lower than the maximum residues limits tolerated for these compounds in milk, the recoveries ranging from 81 to 110%, indicating the potential of the CZE- MS/MS for the analysis of regulated quinolone antibiotics in the food quality and safety control areas. |
类型 | Article |
语种 | 英语 |
国家 | Spain |
收录类别 | SCI-E |
WOS记录号 | WOS:000242021400009 |
WOS关键词 | SOLID-PHASE EXTRACTION ; PERFORMANCE LIQUID-CHROMATOGRAPHY ; FLUORESCENCE DETECTION ; AQUACULTURED PRODUCTS ; ULTRAVIOLET DETECTION ; ANTIBACTERIAL AGENTS ; ANTIMICROBIAL AGENTS ; CHICKEN MUSCLE ; SAMPLES ; FLUOROQUINOLONES |
WOS类目 | Chemistry, Analytical |
WOS研究方向 | Chemistry |
资源类型 | 期刊论文 |
条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/150688 |
作者单位 | (1)Univ Granada, Fac Sci, Dept Analyt Chem, E-18071 Granada, Spain;(2)Puleva Biotech SA, Dept Qual Control, E-18004 Granada, Spain |
推荐引用方式 GB/T 7714 | Lara, Francisco J.,Garcia-Campana, Ana M.,Ales-Barrero, Fermin,et al. Multiresidue method for the determination of quinolone antibiotics in bovine raw milk by capillary electrophoresis-tandem mass spectrometry[J],2006,78(22):7665-7673. |
APA | Lara, Francisco J.,Garcia-Campana, Ana M.,Ales-Barrero, Fermin,Bosque-Sendra, Juan M.,&Garcia-Ayuso, Luis E..(2006).Multiresidue method for the determination of quinolone antibiotics in bovine raw milk by capillary electrophoresis-tandem mass spectrometry.ANALYTICAL CHEMISTRY,78(22),7665-7673. |
MLA | Lara, Francisco J.,et al."Multiresidue method for the determination of quinolone antibiotics in bovine raw milk by capillary electrophoresis-tandem mass spectrometry".ANALYTICAL CHEMISTRY 78.22(2006):7665-7673. |
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